| Literature DB >> 15992607 |
S Pandolfi1, M Bonafè, L Di Tella, L Tiberi, S Salvioli, D Monti, S Sorbi, C Franceschi.
Abstract
p66(shc-/-) mice exhibit prolonged lifespan and increased resistance to oxidative and hypoxic stress. To investigate p66(shc) involvement in human longevity, p66(shc) mRNA and protein were evaluated in fibroblasts from young people, elderly and centenarians, exposed to oxidative or hypoxic stress. Unexpectedly, centenarians showed the highest basal levels of p66(shc). Oxidative stress induced p66(shc) in all samples. At variance, hypoxic stress caused p66(shc) reduction only in cells from centenarians. These changes occurred in absence of any modification of p66(shc) promoter methylation pattern. Intriguingly, in cells from centenarians, p66(shc) induction was affected by p53 codon 72 polymorphism. Thus, cells from centenarians present a peculiar regulation of p66(shc), suggesting that its role in mammalian longevity is more complex than previously thought.Entities:
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Year: 2005 PMID： 15992607 DOI： 10.1016/j.mad.2005.03.004
Source DB: PubMed Journal: Mech Ageing Dev ISSN： 0047-6374 Impact factor: 5.432