Literature DB >> 15988984

Determination of [S,S']-ethylenediamine disuccinic acid (EDDS) by high performance liquid chromatography after derivatization with FMOC.

Susan Tandy1, Rainer Schulin, Marc J F Suter, Bernd Nowack.   

Abstract

The paper describes a new HPLC method for the determination of ethylenediamine disuccinic acid (EDDS). EDDS is derivatized with FMOC reagent followed by HPLC separation on a reversed-phase column. The eluents consist of phosphate buffer at pH 6.8 and acetonitrile. Separation was carried out using gradient elution. The FMOC-EDDS derivative is detected with a fluorescence detector with an excitation wavelength of 265 nm and an emission wavelength of 313 nm. The detection limit is 0.01 microM. The method is applicable to the determination of the compound in water, soil solution and plant material at trace levels.

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Year:  2005        PMID: 15988984     DOI: 10.1016/j.chroma.2005.04.064

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN: 0021-9673            Impact factor:   4.759


  2 in total

1.  Modulation of protein behavior through light responses of TiO2 nanodots films.

Authors:  Kui Cheng; Yi Hong; Mengfei Yu; Jun Lin; Wenjian Weng; Huiming Wang
Journal:  Sci Rep       Date:  2015-08-26       Impact factor: 4.379

2.  High Performance Liquid Chromatography Coupled with Pre-column Derivatization for Determination of Oxidized Glutathione Level in Rats Exposed to Paraquat.

Authors:  Zahra Hami; Mohsen Amini; Amir Kiani; Mahmoud Ghazi-Khansari
Journal:  Iran J Pharm Res       Date:  2013       Impact factor: 1.696

  2 in total

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