Literature DB >> 15986159

Pitfalls and their remedies in time-resolved fluorescence spectroscopy and microscopy.

Martin vandeVen1, Marcel Ameloot, Bernard Valeur, Noël Boens.   

Abstract

Time-resolved fluorescence spectroscopy and microscopy in both time and frequency domains provide very useful and accurate information on dynamic processes. Good quality data are essential in obtaining reliable parameter estimates. Distortions of the fluorescence response due to artifacts may have disastrous consequences. We provide here a concise overview of potential difficulties encountered under daily laboratory circumstances in the use of time- and frequency-domain equipment as well as practical remedies against common error conditions, elucidated with several graphs to aid the researcher in visual inspection and quality-control of collected data. A range of artifacts due to sample preparation or to optical and electronic pitfalls are discussed, as are remedies against them. Also recommended data analysis strategies are described.

Entities:  

Year:  2005        PMID： 15986159     DOI： 10.1007/s10895-005-2632-1

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  13 in total

1.  Fluorescence lifetime-resolved imaging: measuring lifetimes in an image.

Authors:  Robert M Clegg; Oliver Holub; Christopher Gohlke
Journal:  Methods Enzymol       Date:  2003       Impact factor: 1.600

2.  Steady-state fluorescence imaging of neoplasia.

Authors:  Erin M Gill; Gregory M Palmer; Nirmala Ramanujam
Journal:  Methods Enzymol       Date:  2003       Impact factor: 1.600

3.  Fluorescence: basic concepts, practical aspects, and some anecdotes.

Authors:  David M Jameson; John C Croney; Pierre D J Moens
Journal:  Methods Enzymol       Date:  2003       Impact factor: 1.600

4.  Evaluation of global analysis algorithms for single frequency fluorescence lifetime imaging microscopy data.

Authors:  P J Verveer; P I H Bastiaens
Journal:  J Microsc       Date:  2003-01       Impact factor: 1.758

5.  Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM).

Authors:  Andrew H A Clayton; Quentin S Hanley; Donna J Arndt-Jovin; Vinod Subramaniam; Thomas M Jovin
Journal:  Biophys J       Date:  2002-09       Impact factor: 4.033

6.  Time-resolved emission spectra of hemoglobin on the picosecond time scale.

Authors:  E Bucci; H Malak; C Fronticelli; I Gryczynski; G Laczko; J R Lakowicz
Journal:  Biophys Chem       Date:  1988-12       Impact factor: 2.352

7.  Phase fluorometric method for determination of standard lifetimes.

Authors:  R B Thompson; E Gratton
Journal:  Anal Chem       Date:  1988-04-01       Impact factor: 6.986

8.  Maximum entropy method of data analysis in time-resolved spectroscopy.

Authors:  J C Brochon
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

9.  A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution.

Authors:  E Gratton; M Limkeman
Journal:  Biophys J       Date:  1983-12       Impact factor: 4.033

10.  Detecting and minimizing zinc contamination in physiological solutions.

Authors:  Alan R Kay
Journal:  BMC Physiol       Date:  2004-03-15
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  7 in total

1.  Analysis of variance in spectroscopic imaging data from human tissues.

Authors:  Jin Tae Kwak; Rohith Reddy; Saurabh Sinha; Rohit Bhargava
Journal:  Anal Chem       Date:  2011-12-28       Impact factor: 6.986

2.  Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry.

Authors:  Kristin A Fletcher; Sayo O Fakayode; Mark Lowry; Sheryl A Tucker; Sharon L Neal; Irene W Kimaru; Matthew E McCarroll; Gabor Patonay; Philip B Oldham; Oleksandr Rusin; Robert M Strongin; Isiah M Warner
Journal:  Anal Chem       Date:  2006-06-15       Impact factor: 6.986

3.  Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy.

Authors:  Noël Boens; Wenwu Qin; Nikola Basarić; Johan Hofkens; Marcel Ameloot; Jacques Pouget; Jean-Pierre Lefèvre; Bernard Valeur; Enrico Gratton; Martin vandeVen; Norberto D Silva; Yves Engelborghs; Katrien Willaert; Alain Sillen; Garry Rumbles; David Phillips; Antonie J W G Visser; Arie van Hoek; Joseph R Lakowicz; Henryk Malak; Ignacy Gryczynski; Arthur G Szabo; Don T Krajcarski; Naoto Tamai; Atsushi Miura
Journal:  Anal Chem       Date:  2007-02-01       Impact factor: 6.986

4.  Efficient isolation of Pseudomonas aeruginosa type III secretion translocators and assembly of heteromeric transmembrane pores in model membranes.

Authors:  Fabian B Romano; Kyle C Rossi; Christos G Savva; Andreas Holzenburg; Eugenia M Clerico; Alejandro P Heuck
Journal:  Biochemistry       Date:  2011-07-26       Impact factor: 3.162

5.  A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging.

Authors:  Simon P Poland; Nikola Krstajić; James Monypenny; Simao Coelho; David Tyndall; Richard J Walker; Viviane Devauges; Justin Richardson; Neale Dutton; Paul Barber; David Day-Uei Li; Klaus Suhling; Tony Ng; Robert K Henderson; Simon M Ameer-Beg
Journal:  Biomed Opt Express       Date:  2015-01-06       Impact factor: 3.732

6.  In vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADH.

Authors:  Mohammad A Yaseen; Sava Sakadžić; Weicheng Wu; Wolfgang Becker; Karl A Kasischke; David A Boas
Journal:  Biomed Opt Express       Date:  2013-01-22       Impact factor: 3.732

7.  Fluorescence lifetime imaging of molecular rotors in living cells.

Authors:  Klaus Suhling; James A Levitt; Pei-Hua Chung; Marina K Kuimova; Gokhan Yahioglu
Journal:  J Vis Exp       Date:  2012-02-09       Impact factor: 1.355
  7 in total

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