| Literature DB >> 15984012 |
Charlotte Stenh1, Hillevi Englund, Anna Lord, Ann-Sofi Johansson, Claudia G Almeida, Pär Gellerfors, Paul Greengard, Gunnar K Gouras, Lars Lannfelt, Lars N G Nilsson.
Abstract
Amyloid-beta (Abeta) peptide levels are widely measured by enzyme-linked immunosorbent assay (ELISA) in Alzheimer's disease research. Here, we show that oligomerization of Abeta results in underestimated Abeta ELISA levels. The implications are that comprehensive analysis of soluble Abeta requires either sample pretreatment at denaturing conditions or novel conformation-dependent immunoassays. Our findings might be of relevance for many neurodegenerative disorders in which soluble protein aggregates are the main neurotoxic species.Entities:
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Year: 2005 PMID： 15984012 DOI： 10.1002/ana.20524
Source DB: PubMed Journal: Ann Neurol ISSN： 0364-5134 Impact factor: 10.422