| Literature DB >> 15979747 |
Manuela Grassi1, Elisabetta Volpe, Vittorio Colizzi, Francesca Mariani.
Abstract
The detection of low abundance mRNA and/or GC-rich targets is very difficult using real-time PCR, often requiring laborious optimization procedures. This work shows that formamide is a useful PCR additive, increasing the sensitivity and specificity of SYBR Green real-time PCR to detect low abundance mycobacterial RNA from infected samples.Entities:
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Year: 2005 PMID： 15979747 DOI： 10.1016/j.mimet.2005.05.002
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363