OBJECTIVES: The very-low-density lipoprotein receptor (VLDLr) is highly expressed in macrophage-rich areas of atherosclerotic lesions. The exact role of the macrophage VLDLr in atherosclerotic lesion development, however, is presently unclear. METHODS AND RESULTS: To assess the role of the macrophage VLDLr in atherosclerotic lesion development in vivo, we used the technique of bone marrow transplantation to selectively disrupt or reconstitute the VLDLr in macrophages in VLDLr+/+ and VLDLr-/- mice, respectively. After 10 weeks high-cholesterol diet feeding, the lesion area in control transplanted wild-type mice was 17+/-4 x 10(3)+/-microm(2). Disruption of the macrophage VLDLr by transplanting bone marrow from VLDLr-/- mice to wild-type VLDLr+/+ littermates resulted in a tendency to a slight reduction in lesion size to 12+/-3 x 10 microm. The mean atherosclerotic lesion area, measured in control transplanted VLDLr-/- mice, lacking the VLDLr in all tissues was 12+/-3 x 10(3)microm(2). Interestingly, reconstitution of the macrophage VLDLr in VLDLr-deficient recipients resulted in a 2.7-fold increase (P<0.05) in the mean atherosclerotic lesion area to 32+/-3 x 10(3)microm(2). CONCLUSIONS: The macrophage VLDLr facilitates atherosclerotic lesion development, probably by mediating the accumulation of atherogenic lipoproteins.
OBJECTIVES: The very-low-density lipoprotein receptor (VLDLr) is highly expressed in macrophage-rich areas of atherosclerotic lesions. The exact role of the macrophage VLDLr in atherosclerotic lesion development, however, is presently unclear. METHODS AND RESULTS: To assess the role of the macrophage VLDLr in atherosclerotic lesion development in vivo, we used the technique of bone marrow transplantation to selectively disrupt or reconstitute the VLDLr in macrophages in VLDLr+/+ and VLDLr-/- mice, respectively. After 10 weeks high-cholesterol diet feeding, the lesion area in control transplanted wild-type mice was 17+/-4 x 10(3)+/-microm(2). Disruption of the macrophage VLDLr by transplanting bone marrow from VLDLr-/- mice to wild-type VLDLr+/+ littermates resulted in a tendency to a slight reduction in lesion size to 12+/-3 x 10 microm. The mean atherosclerotic lesion area, measured in control transplanted VLDLr-/- mice, lacking the VLDLr in all tissues was 12+/-3 x 10(3)microm(2). Interestingly, reconstitution of the macrophage VLDLr in VLDLr-deficient recipients resulted in a 2.7-fold increase (P<0.05) in the mean atherosclerotic lesion area to 32+/-3 x 10(3)microm(2). CONCLUSIONS: The macrophage VLDLr facilitates atherosclerotic lesion development, probably by mediating the accumulation of atherogenic lipoproteins.
Authors: Yinyuan Ding; Linzhang Huang; Xunde Xian; Ivan S Yuhanna; Catherine R Wasser; Michael Frotscher; Chieko Mineo; Philip W Shaul; Joachim Herz Journal: Sci Signal Date: 2016-03-15 Impact factor: 8.192
Authors: Daniel Baitsch; Hans H Bock; Thomas Engel; Ralph Telgmann; Carsten Müller-Tidow; Georg Varga; Martine Bot; Joachim Herz; Horst Robenek; Arnold von Eckardstein; Jerzy-Roch Nofer Journal: Arterioscler Thromb Vasc Biol Date: 2011-02-24 Impact factor: 8.311
Authors: Karin Fredriksson; Amarjit Mishra; Jonathan K Lam; Elizabeth M Mushaben; Rosemarie A Cuento; Katharine S Meyer; Xianglan Yao; Karen J Keeran; Gayle Z Nugent; Xuan Qu; Zu-Xi Yu; Yanqin Yang; Nalini Raghavachari; Pradeep K Dagur; J Philip McCoy; Stewart J Levine Journal: J Immunol Date: 2014-04-14 Impact factor: 5.422