Karin Przyklenk1, Peter Whittaker. 1. Departments of Emergency Medicine and Anesthesiology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655, USA. Karin.Przyklenk@umassmed.edu
Abstract
BACKGROUND: Brief preconditioning (PC) ischemia, in addition to its well-described cardioprotective effects, has been shown in some studies to act on circulating platelets and attenuate platelet adhesion and aggregation in models of unstable angina and acute myocardial infarction. This "anti-platelet" effect of PC may be triggered by release of adenosine from ischemic/reperfused myocardium and activation of adenosine A(2) receptors on the platelets' surface. However: (1) all current data on the platelet inhibitory effects of PC ischemia and A(2) receptor stimulation have been obtained in adult populations; and (2) there is evidence of age-associated alterations in myocardial adenosine release, receptor responsiveness and post-receptor signaling. OBJECTIVE: Our aim was to evaluate, using an established in vitro model of platelet aggregation and exogenous administration of an adenosine A(2) agonist, whether the favorable effects of adenosine A(2) receptor stimulation on platelet responsiveness are compromised in aging populations. METHODS: Arterial blood samples were obtained from young adult versus old rabbits (6 months versus 4 years of age) and young adult versus senescent rats (4 months versus 2 years of age). Matched aliquots from each animal were randomly assigned to receive exogenous treatment with either the A(2) agonist CGS 21680 or vehicle. Maximum platelet aggregation was quantified by whole blood impedance aggregometry, using collagen as the aggregatory stimulus. RESULTS: In young adult rabbits, maximum platelet aggregation was, as expected, reduced by 30 +/- 4% in CGS-treated aliquots versus vehicle-controls. In contrast, blood samples from 4 year old rabbits were refractory to A(2) receptor stimulation: in the old cohort, treatment with CGS evoked no change in platelet aggregation (decrease of 2 +/- 3% versus age-matched vehicle controls; p < .01 versus the decrease of 30% seen in young adults). Data obtained in the rat model were analogous to those seen in the rabbit: maximum platelet aggregation decreased by 18 +/- 5% versus 1 +/- 7% with CGS treatment in young adult versus senescent animals. CONCLUSION: Our results provide novel in vitro evidence of an age-associated loss in platelet responsiveness to adenosine-mediated "preconditioning".
BACKGROUND: Brief preconditioning (PC) ischemia, in addition to its well-described cardioprotective effects, has been shown in some studies to act on circulating platelets and attenuate platelet adhesion and aggregation in models of unstable angina and acute myocardial infarction. This "anti-platelet" effect of PC may be triggered by release of adenosine from ischemic/reperfused myocardium and activation of adenosine A(2) receptors on the platelets' surface. However: (1) all current data on the platelet inhibitory effects of PCischemia and A(2) receptor stimulation have been obtained in adult populations; and (2) there is evidence of age-associated alterations in myocardial adenosine release, receptor responsiveness and post-receptor signaling. OBJECTIVE: Our aim was to evaluate, using an established in vitro model of platelet aggregation and exogenous administration of an adenosine A(2) agonist, whether the favorable effects of adenosine A(2) receptor stimulation on platelet responsiveness are compromised in aging populations. METHODS: Arterial blood samples were obtained from young adult versus old rabbits (6 months versus 4 years of age) and young adult versus senescent rats (4 months versus 2 years of age). Matched aliquots from each animal were randomly assigned to receive exogenous treatment with either the A(2) agonist CGS 21680 or vehicle. Maximum platelet aggregation was quantified by whole blood impedance aggregometry, using collagen as the aggregatory stimulus. RESULTS: In young adult rabbits, maximum platelet aggregation was, as expected, reduced by 30 +/- 4% in CGS-treated aliquots versus vehicle-controls. In contrast, blood samples from 4 year old rabbits were refractory to A(2) receptor stimulation: in the old cohort, treatment with CGS evoked no change in platelet aggregation (decrease of 2 +/- 3% versus age-matched vehicle controls; p < .01 versus the decrease of 30% seen in young adults). Data obtained in the rat model were analogous to those seen in the rabbit: maximum platelet aggregation decreased by 18 +/- 5% versus 1 +/- 7% with CGS treatment in young adult versus senescent animals. CONCLUSION: Our results provide novel in vitro evidence of an age-associated loss in platelet responsiveness to adenosine-mediated "preconditioning".
Authors: John P Headrick; Laura Willems; Kevin J Ashton; Kirsten Holmgren; Jason Peart; G Paul Matherne Journal: J Physiol Date: 2003-04-25 Impact factor: 5.182
Authors: Boris Z Simkhovich; Paul Marjoram; Coralie Poizat; Larry Kedes; Robert A Kloner Journal: Arch Biochem Biophys Date: 2003-12-15 Impact factor: 4.013
Authors: P Abete; N Ferrara; A Cioppa; P Ferrara; S Bianco; C Calabrese; F Cacciatore; G Longobardi; F Rengo Journal: J Am Coll Cardiol Date: 1996-06 Impact factor: 24.094
Authors: J D Emery; D W Leifer; G L Moura; P Southern; J H Morrissey; J B Lawrence Journal: Arterioscler Thromb Vasc Biol Date: 1995-06 Impact factor: 8.311