| Literature DB >> 15967475 |
Amy L Haas1, Nathan P Laun, Tadhg P Begley.
Abstract
Saccharomyces cerevisiae Thi20 is a fusion protein with homology to Bacillus subtilis ThiD and TenA. The N-terminus of Thi20 has significant sequence homology to B. subtilis ThiD, while the C-terminus has homology to B. subtilis TenA. Incubation of Thi20 with thiamin reveals that it has thiaminase II activity, in addition, incubation of Thi20 with HMP (4-amino-2-methyl-5-hydroxymethylpyrimidine) and ATP reveals that it has HMP kinase and HMP-P (4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate) kinase activity. This demonstrates that Thi20 is a trifunctional protein with thiamin biosynthetic and degradative activity.Entities:
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Year: 2005 PMID： 15967475 DOI： 10.1016/j.bioorg.2005.04.001
Source DB: PubMed Journal: Bioorg Chem ISSN： 0045-2068 Impact factor: 5.275