Literature DB >> 1596361

Construction of a plasmid containing the complete coding region of human elongation factor 2.

J Hanes1, J Freudenstein, G Rapp, K H Scheit.   

Abstract

A plasmid pUChEF-2 containing the coding sequence as well as the complete 3'-untranslated region (3'UTR) of human EF-2 mRNA was constructed. The plasmid construct was assembled from a cDNA insert of pHGR81 (Rapp et al., (1988) Biol. Chem. Hoppe-Seyler 369, 247-250) comprising the C-terminal portion of the coding region and the 3'UTR, as well as a polymer chain reaction PCR fragment (Rapp et al., (1989) Biol. Chem. Hoppe-Seyler 370, 1071-1075) covering the missing part of the coding region from the amino-terminus.

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Year:  1992        PMID： 1596361     DOI： 10.1515/bchm3.1992.373.1.201

Source DB:  PubMed          Journal:  Biol Chem Hoppe Seyler        ISSN： 0177-3593


  2 in total

1.  The 5' terminal oligopyrimidine tract confers translational control on TOP mRNAs in a cell type- and sequence context-dependent manner.

Authors:  D Avni; Y Biberman; O Meyuhas
Journal:  Nucleic Acids Res       Date:  1997-03-01       Impact factor: 16.971

2.  Identification of anti-Gram-negative bacteria agents targeting the interaction between ribosomal proteins L12 and L10.

Authors:  Weiwei Wang; Chao Liu; Ningyu Zhu; Yuan Lin; Jiandong Jiang; Yanchang Wang; Yan Li; Shuyi Si
Journal:  Acta Pharm Sin B       Date:  2018-07-30       Impact factor: 11.413
  2 in total

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