Jian Song1, Dong-Sheng Zhang, Jie Zheng. 1. Department of Pathology, Medical School of Southeast University, Nanjing 210009, Jiangsu Province, China.
Abstract
AIM: To investigate if CD44v6 could be used as a molecular marker of cancer progression and metastasis through the detection of CD44v6 gene expression in normal human peripheral blood. METHODS: RNA was extracted from the peripheral blood mononuclear cells of 50 healthy donors, the expression of CD44v6 was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: CD44v6 mRNA was detected in 58% of healthy volunteers under the proper controls. CONCLUSION: Our results suggest that the measurement of CD44v6 expression in peripheral blood by RT-PCR is not suitable for detection of circulating tumor cells.
AIM: To investigate if CD44v6 could be used as a molecular marker of cancer progression and metastasis through the detection of CD44v6 gene expression in normal human peripheral blood. METHODS: RNA was extracted from the peripheral blood mononuclear cells of 50 healthy donors, the expression of CD44v6 was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: CD44v6 mRNA was detected in 58% of healthy volunteers under the proper controls. CONCLUSION: Our results suggest that the measurement of CD44v6 expression in peripheral blood by RT-PCR is not suitable for detection of circulating tumor cells.
Authors: U Günthert; M Hofmann; W Rudy; S Reber; M Zöller; I Haussmann; S Matzku; A Wenzel; H Ponta; P Herrlich Journal: Cell Date: 1991-04-05 Impact factor: 41.582
Authors: Andrew P Barbour; Jennifer A Reeder; Michael D Walsh; Jonathan Fawcett; Toni M Antalis; David C Gotley Journal: Cancer Res Date: 2003-02-15 Impact factor: 12.701
Authors: H Schrewe; J Thompson; M Bona; L J Hefta; A Maruya; M Hassauer; J E Shively; S von Kleist; W Zimmermann Journal: Mol Cell Biol Date: 1990-06 Impact factor: 4.272
Authors: L Hefler; C Tempfer; G Haeusler; E Kucera; K Mayerhofer; R Zeillinger; A Reinthaller; C Kainz Journal: Int J Cancer Date: 1998-10-23 Impact factor: 7.396
Authors: S Goodison; K Yoshida; T Sugino; A Woodman; H Gorham; J Bolodeoku; M Kaufmann; D Tarin Journal: Cancer Res Date: 1997-08-01 Impact factor: 12.701