Literature DB >> 15956586

A functional histidine-tagged replication initiator protein: implications for the study of single-stranded DNA virus replication in planta.

Julio C Vega-Arreguín1, Tatiana Timchenko, Bruno Gronenborn, Bertha Cecilia Ramírez.   

Abstract

Replication initiation of nanoviruses, plant viruses with a multipartite circular single-stranded DNA genome, is triggered by the master Rep (M-Rep) protein. To enable the study of interactions between M-Rep and viral or host factors involved in replication, we designed oligohistidine-tagged variants of the nanovirus Faba bean necrotic yellows virus (FBNYV) M-Rep protein that allow affinity purification of enzymatically active M-Rep from plant tissue. The tagged M-Rep protein was able to initiate replication of its cognate and other FBNYV DNAs in Nicotiana benthamiana leaf disks and plants. The replicon encoding the tagged M-Rep protein multiplied and moved systemically in FBNYV-infected Vicia faba plants and was transmitted by the aphid vector of the virus. Using the tagged M-Rep protein, we demonstrated the in planta interaction between wild-type M-Rep and its tagged counterpart. Such a tagged and fully functional replication initiator protein will have bearings on the isolation of protein complexes from plants.

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Year:  2005        PMID： 15956586      PMCID： PMC1143747          DOI： 10.1128/JVI.79.13.8422-8430.2005

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  40 in total

1.  Clink, a nanovirus-encoded protein, binds both pRB and SKP1.

Authors:  M N Aronson; A D Meyer; J Györgyey; L Katul; H J Vetten; B Gronenborn; T Timchenko
Journal:  J Virol       Date:  2000-04       Impact factor: 5.103

2.  Viral movement proteins as probes for intracellular and intercellular trafficking in plants

Authors: 
Journal:  Plant Cell       Date:  1999-04       Impact factor: 11.277

3.  The master rep concept in nanovirus replication: identification of missing genome components and potential for natural genetic reassortment.

Authors:  T Timchenko; L Katul; Y Sano; F de Kouchkovsky; H J Vetten; B Gronenborn
Journal:  Virology       Date:  2000-08-15       Impact factor: 3.616

4.  In planta protein-protein interactions assessed using a nanovirus-based replication and expression system.

Authors:  Marie N Aronson; Arnaud Complainville; Danielle Clérot; Hélène Alcalde; Lina Katul; H Josef Vetten; Bruno Gronenborn; Tatiana Timchenko
Journal:  Plant J       Date:  2002-09       Impact factor: 6.417

5.  The adeno-associated virus Rep78 major regulatory protein forms multimeric complexes and the domain for this activity is contained within the carboxy-half of the molecule.

Authors:  P L Hermonat; R B Batchu
Journal:  FEBS Lett       Date:  1997-01-20       Impact factor: 4.124

6.  Octopine Ti-plasmid deletion mutants of agrobacterium tumefaciens with emphasis on the right side of the T-region.

Authors:  G Ooms; P J Hooykaas; R J Van Veen; P Van Beelen; T J Regensburg-Tuïnk; R A Schilperoort
Journal:  Plasmid       Date:  1982-01       Impact factor: 3.466

7.  A single rep protein initiates replication of multiple genome components of faba bean necrotic yellows virus, a single-stranded DNA virus of plants.

Authors:  T Timchenko; F de Kouchkovsky; L Katul; C David; H J Vetten; B Gronenborn
Journal:  J Virol       Date:  1999-12       Impact factor: 5.103

8.  Faba bean necrotic yellows virus (genus Nanovirus) requires a helper factor for its aphid transmission.

Authors:  A W Franz; F van der Wilk; M Verbeek; A M Dullemans; J F van den Heuvel
Journal:  Virology       Date:  1999-09-15       Impact factor: 3.616

9.  The multifunctional character of a geminivirus replication protein is reflected by its complex oligomerization properties.

Authors:  B M Orozco; L J Kong; L A Batts; S Elledge; L Hanley-Bowdoin
Journal:  J Biol Chem       Date:  2000-03-03       Impact factor: 5.157

10.  Ten distinct circular ssDNA components, four of which encode putative replication-associated proteins, are associated with the faba bean necrotic yellows virus genome.

Authors:  L Katul; T Timchenko; B Gronenborn; H J Vetten
Journal:  J Gen Virol       Date:  1998-12       Impact factor: 3.891
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  3 in total

1.  Recent advances in understanding the replication initiator protein of the ssDNA plant viruses of the family Nanoviridae.

Authors:  Sangita Venkataraman; R Selvarajan
Journal:  Virusdisease       Date:  2019-03-01

2.  The nanovirus-encoded Clink protein affects plant cell cycle regulation through interaction with the retinoblastoma-related protein.

Authors:  Sébastien Lageix; Olivier Catrice; Jean-Marc Deragon; Bruno Gronenborn; Thierry Pélissier; Bertha Cecilia Ramírez
Journal:  J Virol       Date:  2007-01-31       Impact factor: 5.103

3.  A multicellular way of life for a multipartite virus.

Authors:  Anne Sicard; Elodie Pirolles; Romain Gallet; Marie-Stéphanie Vernerey; Michel Yvon; Cica Urbino; Michel Peterschmitt; Serafin Gutierrez; Yannis Michalakis; Stéphane Blanc
Journal:  Elife       Date:  2019-03-12       Impact factor: 8.140
  3 in total

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