| Literature DB >> 15955446 |
Monika Paul-Samojedny1, Danuta Kokocińska, Arkadiusz Samojedny, Urszula Mazurek, Robert Partyka, Zbigniew Lorenz, Tadeusz Wilczok.
Abstract
The rate of tumour growth is dependent on the balance between proliferation and apoptosis at all stages of carcinogenesis. Apoptosis inhibition, in turn, depends partly on the balance between expression of two cell death regulatory genes, Bcl-2 and Bax. Colon cancer has long been associated with disturbances in apoptosis regulation. The aim of our study was to determine the expression levels of Bcl-2 and Bax mRNAs in 1 microg sample of total RNA obtained from normal colon and colon adenocarcinoma. This study was intended to evaluate possible differences in Bcl-2 and Bax mRNA levels at particular stages of colon adenocarcinoma classified according to Duke's system. The apoptotic frequency (represented by Bax mRNA copy number) was inversely proportional to the decrease of Bcl-2 gene expression. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) was performed to confirm apoptosis.Entities:
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Year: 2004 PMID： 15955446 DOI： 10.1016/j.bbadis.2004.11.021
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002