| Literature DB >> 15955329 |
Alfonso Olivos1, Espiridión Ramos, Mario Nequiz, Carlos Barba, Eusebio Tello, Guadalupe Castañón, Augusto González, Rubén D Martínez, Irmgard Montfort, Ruy Pérez-Tamayo.
Abstract
Intraportal injection of non-virulent E. histolytica (derived from prolonged axenic culture of virulent E. histolytica) strain HM1-IMSS in normal hamsters results in no liver lesions and disappearance of the parasites 48-72 h after injection. Viability of non-virulent E. histolytica after 2 h of in vitro incubation in either fresh or decomplemented hamster serum is the same as control virulent E. histolytica (50-90%). In hamsters made leukopenic, or both leukopenic+hypocomplementemic, or hypocomplementemic+sephadex microspheres (to produce focal liver ischemia) intraportally injected non-virulent E. histolytica cause no lesions and disappear after 24 h. In addition, neither hypocomplementemia nor immunosuppression with cyclosporin A prolonged the survival of non-virulent E. histolytica. Methyl prednisolone treatment of hamsters resulted in survival of large numbers of non-virulent E. histolytica in the liver, with little inflammation and minimal tissue damage, for up to 7 days. Inflammatory cells (macrophages) would appear to be chiefly responsible for elimination of non-virulent E. histolytica. Parallel experiments with E. dispar suggest a different mechanism for its non-pathogenicity.Entities:
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Year: 2005 PMID： 15955329 DOI： 10.1016/j.exppara.2005.03.020
Source DB: PubMed Journal: Exp Parasitol ISSN： 0014-4894 Impact factor: 2.011