OBJECTIVE: To describe microbiological characteristics and epidemiologic features of an outbreak of postpartum endometritis. METHODS: Various markers were investigated in five patients and three throat carriage isolates of Streptococcus pyogenes obtained during an outbreak of endometritis occurring in a 13-week period. Molecular characterization included biotyping, T-serotyping, emm gene sequence and restriction, pulsed-field gel electrophoresis (PFGE), and random amplified polymorphic DNA (RAPD) analysis. RESULTS: Biotype, T-serotype, and genotypic data (emm analysis, PFGE, and RAPD analysis) revealed a close relationship among the isolates from three patients, suggesting that cross-contamination had occurred. These isolates were biotype 1, T type 28, and emm type 28. The isolates from one patient and one carrier differed from those of the index patient by minor variations of the emm amplicon restriction pattern, PFGE pattern, or RAPD pattern. The remaining isolates were phenotypically and genetically different. CONCLUSION: Identification of different isolates demonstrated that different strains may circulate simultaneously during a true outbreak and that the predominant strain might persist for several months.
OBJECTIVE: To describe microbiological characteristics and epidemiologic features of an outbreak of postpartum endometritis. METHODS: Various markers were investigated in five patients and three throat carriage isolates of Streptococcus pyogenes obtained during an outbreak of endometritis occurring in a 13-week period. Molecular characterization included biotyping, T-serotyping, emm gene sequence and restriction, pulsed-field gel electrophoresis (PFGE), and random amplified polymorphic DNA (RAPD) analysis. RESULTS: Biotype, T-serotype, and genotypic data (emm analysis, PFGE, and RAPD analysis) revealed a close relationship among the isolates from three patients, suggesting that cross-contamination had occurred. These isolates were biotype 1, T type 28, and emm type 28. The isolates from one patient and one carrier differed from those of the index patient by minor variations of the emm amplicon restriction pattern, PFGE pattern, or RAPD pattern. The remaining isolates were phenotypically and genetically different. CONCLUSION: Identification of different isolates demonstrated that different strains may circulate simultaneously during a true outbreak and that the predominant strain might persist for several months.
Authors: A Lepoutre; A Doloy; P Bidet; A Leblond; A Perrocheau; E Bingen; P Trieu-Cuot; A Bouvet; C Poyart; D Lévy-Bruhl Journal: J Clin Microbiol Date: 2011-10-05 Impact factor: 5.948
Authors: Bogdan Luca-Harari; Kim Ekelund; Mark van der Linden; Margit Staum-Kaltoft; Anette M Hammerum; Aftab Jasir Journal: J Clin Microbiol Date: 2007-10-24 Impact factor: 5.948
Authors: Mark J Walker; Timothy C Barnett; Jason D McArthur; Jason N Cole; Christine M Gillen; Anna Henningham; K S Sriprakash; Martina L Sanderson-Smith; Victor Nizet Journal: Clin Microbiol Rev Date: 2014-04 Impact factor: 26.132
Authors: Priyanka Kachroo; Jesus M Eraso; Stephen B Beres; Randall J Olsen; Luchang Zhu; Waleed Nasser; Paul E Bernard; Concepcion C Cantu; Matthew Ojeda Saavedra; María José Arredondo; Benjamin Strope; Hackwon Do; Muthiah Kumaraswami; Jaana Vuopio; Kirsi Gröndahl-Yli-Hannuksela; Karl G Kristinsson; Magnus Gottfredsson; Maiju Pesonen; Johan Pensar; Emily R Davenport; Andrew G Clark; Jukka Corander; Dominique A Caugant; Shahin Gaini; Marita Debess Magnussen; Samantha L Kubiak; Hoang A T Nguyen; S Wesley Long; Adeline R Porter; Frank R DeLeo; James M Musser Journal: Nat Genet Date: 2019-02-18 Impact factor: 38.330