OBJECTIVE: To investigate the distribution of fimA genotype of P. gingivalis in chronic periodontitis patients. METHODS: Subgingival plaque samples were collected from 101 chronic periodontitis patients. P. gingivalis was detected by both culture method and P. gingivalis 16S rRNA PCR. fimA type-specific primer were designed, and the distribution of fimA genotype of P. gingivalis in periodontitis patients were detected by PCR. RESULTS: The detective ratio of P. gingivalis was 88.1%. Among them, a single fimA genotype was detected in most subgingival plaque samples (65.1%), and the distribution of five fimA genotypes among P. gingivalis positive patients was as follows: type I, 24.7%; type II, 43.8%; type III, 15.7%; type IV, 40.4%; type V, 3.4%; respectively. CONCLUSION: P. gingivalis with various fimA genotypes were present in subgingival plaque samples from chronic periodontitis patients, and P. gingivalis with type II fimA and IV fimA were more predominant in chronic periodontitis patients, and they may be associated with the development of periodontitis.
OBJECTIVE: To investigate the distribution of fimA genotype of P. gingivalis in chronic periodontitispatients. METHODS: Subgingival plaque samples were collected from 101 chronic periodontitispatients. P. gingivalis was detected by both culture method and P. gingivalis 16S rRNA PCR. fimA type-specific primer were designed, and the distribution of fimA genotype of P. gingivalis in periodontitispatients were detected by PCR. RESULTS: The detective ratio of P. gingivalis was 88.1%. Among them, a single fimA genotype was detected in most subgingival plaque samples (65.1%), and the distribution of five fimA genotypes among P. gingivalis positive patients was as follows: type I, 24.7%; type II, 43.8%; type III, 15.7%; type IV, 40.4%; type V, 3.4%; respectively. CONCLUSION:P. gingivalis with various fimA genotypes were present in subgingival plaque samples from chronic periodontitispatients, and P. gingivalis with type II fimA and IV fimA were more predominant in chronic periodontitispatients, and they may be associated with the development of periodontitis.