| Literature DB >> 15950972 |
Mohammed Omedul Islam1, Yonehiro Kanemura, Jesmin Tajria, Hideki Mori, Satoshi Kobayashi, Tomoko Shofuda, Jun Miyake, Masayuki Hara, Mami Yamasaki, Hideyuki Okano.
Abstract
We investigated the localization and functional expression of the ABC transporter ABCB1 in human fetal neural stem/progenitor cells (hNSPCs). RT-PCR analysis revealed ABCB1 gene expression in hNSPCs. We found a single band in immunoblotted hNSPCs lysates probed with ABCB1 antibody, and detected ABCB1 at the hNSPCs cell membrane by immunocytochemistry and subcellular fractionation. ABCB1 inhibitors and substrate, and ATP-depleting agents enhanced hNSPCs' rhodamine 123 accumulation, and hNSPCs microsomes had vanadate-sensitive ATPase activity. ABCB1 and nestin expression decreased during hNSPCs differentiation, while the astroglial marker GFAP increased. ABCB1 may maintain hNSPCs in an undifferentiated state and could be a neural stem/progenitor marker.Entities:
Mesh：
Substances：
Year: 2005 PMID： 15950972 DOI： 10.1016/j.febslet.2005.05.019
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124