| Literature DB >> 15950382 |
Kylie B O'Brien1, Robert F Miller, Michael T Bowser.
Abstract
Uptake of the neuromodulator D-serine by isolated larval tiger salamander (Ambystoma tigrinum) retinas was measured using capillary electrophoresis (CE). Excised retinas were incubated in Ringer's solution in the presence of 5 microM D-serine. The supernatant was removed after 30 min, mixed with 4-fluoro-7-nitrobenz-2-oxa-1,3-diazole (NBD-F) to fluorescently label amines and analyzed using CE. Significant D-serine uptake was observed over a period of 1.5h. This is the first observation of D-serine uptake by an intact retinal tissue. D-Serine uptake in the retina was Na(+)-dependent and blocked by l-alanine, l-threonine, and l-cysteine. This pharmacology is consistent with the sodium dependent heteroexchange expected of system ASC-type transporters.Entities:
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Year: 2005 PMID： 15950382 DOI： 10.1016/j.neulet.2005.05.009
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046