PROBLEM: To determine the levels of cytokines produced upon mitogenic or antigenic stimulation of maternal peripheral blood mononuclear cells (PBMC) from women with pre-eclampsia. METHOD OF STUDY: PBMC from 54 women with a history of successful pregnancy and 32 women undergoing pre-eclamptic delivery were stimulated with a mitogen or with autologous placental cells or with trophoblast antigens, and the levels of cytokines released into the culture supernatants then assessed by enzyme-linked immunosorbent assay. RESULTS: Significantly higher levels of the Th1 cytokines, interferon-gamma, and tumor necrosis factor-alpha were produced by the pre-eclamptic group than by the normal pregnancy group, which on the contrary showed significantly greater production of the Th2 cytokines, interleukin (IL)-4, IL-5, IL-6 and IL-10. A comparison of the ratios of Th2 to Th1 cytokines indicates a higher Th1 cytokine bias in pre-eclampsia as compared with normal pregnancy. CONCLUSIONS: These data are suggestive of a maternal pro-inflammatory cytokine bias in pre-eclampsia. Copyright Blackwell Munksgaard, 2005
PROBLEM: To determine the levels of cytokines produced upon mitogenic or antigenic stimulation of maternal peripheral blood mononuclear cells (PBMC) from women with pre-eclampsia. METHOD OF STUDY: PBMC from 54 women with a history of successful pregnancy and 32 women undergoing pre-eclamptic delivery were stimulated with a mitogen or with autologous placental cells or with trophoblast antigens, and the levels of cytokines released into the culture supernatants then assessed by enzyme-linked immunosorbent assay. RESULTS: Significantly higher levels of the Th1 cytokines, interferon-gamma, and tumor necrosis factor-alpha were produced by the pre-eclamptic group than by the normal pregnancy group, which on the contrary showed significantly greater production of the Th2 cytokines, interleukin (IL)-4, IL-5, IL-6 and IL-10. A comparison of the ratios of Th2 to Th1 cytokines indicates a higher Th1 cytokine bias in pre-eclampsia as compared with normal pregnancy. CONCLUSIONS: These data are suggestive of a maternal pro-inflammatory cytokine bias in pre-eclampsia. Copyright Blackwell Munksgaard, 2005
Authors: Vanessa R Ribeiro; Mariana Romao-Veiga; Graziela G Romagnoli; Mariana L Matias; Priscila R Nunes; Vera Therezinha M Borges; Jose C Peracoli; Maria Terezinha S Peracoli Journal: Immunology Date: 2017-06-19 Impact factor: 7.397
Authors: Jeffrey M Denney; Edward L Nelson; Pathik D Wadhwa; Thaddeus P Waters; Leny Mathew; Esther K Chung; Robert L Goldenberg; Jennifer F Culhane Journal: Cytokine Date: 2010-11-30 Impact factor: 3.861
Authors: Mara Zambruni; Alex Villalobos; Anoma Somasunderam; Sarah Westergaard; Maitreyee Nigalye; Christie G Turin; Jaime Zegarra; Sicilia Bellomo; Erik Mercado; Theresa J Ochoa; Netanya S Utay Journal: J Reprod Immunol Date: 2017-04-06 Impact factor: 4.054