BACKGROUND: The aim of this study was to compare the activity of neutrophilic granulocytes in patients with severe periodontitis and patients with gingivitis alone. METHODS: The study population comprised 22 patients with gingivitis and 44 with periodontitis. Samples of gingival crevicular fluid (GCF) were collected from untreated patients with gingivitis and from shallow and deep pockets in untreated patients with periodontitis. GCF samples were analyzed for lactoferrin, elastase, matrix metalloproteinase-8 and -9, and collagenolytic activity. RESULTS: The free elastase activity and the neutrophil activity, estimated as the ratio between elastase and lactoferrin, were significantly higher in the samples from the periodontitis patients. These differences were also observed in shallow pockets in periodontitis patients compared to similar pockets in patients with gingivitis. CONCLUSION: This study shows higher levels of free elastase in untreated patients with periodontitis, relative to inflammation-matched controls, which may explain the tissue destruction seen in periodontitis.
BACKGROUND: The aim of this study was to compare the activity of neutrophilic granulocytes in patients with severe periodontitis and patients with gingivitis alone. METHODS: The study population comprised 22 patients with gingivitis and 44 with periodontitis. Samples of gingival crevicular fluid (GCF) were collected from untreated patients with gingivitis and from shallow and deep pockets in untreated patients with periodontitis. GCF samples were analyzed for lactoferrin, elastase, matrix metalloproteinase-8 and -9, and collagenolytic activity. RESULTS: The free elastase activity and the neutrophil activity, estimated as the ratio between elastase and lactoferrin, were significantly higher in the samples from the periodontitispatients. These differences were also observed in shallow pockets in periodontitispatients compared to similar pockets in patients with gingivitis. CONCLUSION: This study shows higher levels of free elastase in untreated patients with periodontitis, relative to inflammation-matched controls, which may explain the tissue destruction seen in periodontitis.
Authors: O Laugisch; M Schacht; A Guentsch; T Kantyka; A Sroka; H R Stennicke; W Pfister; A Sculean; J Potempa; S Eick Journal: Mol Oral Microbiol Date: 2011-12-07 Impact factor: 3.563
Authors: Irundika H K Dias; Lindsay Marshall; Peter A Lambert; Iain L C Chapple; John B Matthews; Helen R Griffiths Journal: Infect Immun Date: 2007-11-19 Impact factor: 3.441
Authors: Cecilia Fabiana Márquez-Arrico; Javier Silvestre-Rangil; Laura Gutiérrez-Castillo; Mayte Martinez-Herrera; Francisco Javier Silvestre; Milagros Rocha Journal: J Clin Med Date: 2020-05-23 Impact factor: 4.241