Literature DB >> 15946123

A novel method for observing proteins in vivo using a small fluorescent label and multiphoton imaging.

Stanley W Botchway1, Ignasi Barba, Randolf Jordan, Rebecca Harmston, Peter M Haggie, Simon-Peter Williams, Alexandra M Fulton, Anthony W Parker, Kevin M Brindle.   

Abstract

A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells.

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Year:  2005        PMID： 15946123      PMCID： PMC1199672          DOI： 10.1042/BJ20050648

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  17 in total

Review 1.  Multiphoton microscopy in life sciences.

Authors:  K König
Journal:  J Microsc       Date:  2000-11       Impact factor: 1.758

2.  Multicolor and electron microscopic imaging of connexin trafficking.

Authors:  Guido Gaietta; Thomas J Deerinck; Stephen R Adams; James Bouwer; Oded Tour; Dale W Laird; Gina E Sosinsky; Roger Y Tsien; Mark H Ellisman
Journal:  Science       Date:  2002-04-19       Impact factor: 47.728

3.  A general method for the covalent labeling of fusion proteins with small molecules in vivo.

Authors:  Antje Keppler; Susanne Gendreizig; Thomas Gronemeyer; Horst Pick; Horst Vogel; Kai Johnsson
Journal:  Nat Biotechnol       Date:  2002-12-09       Impact factor: 54.908

4.  The RNA-binding protein Vg1 RBP is required for cell migration during early neural development.

Authors:  Karina Yaniv; Abraham Fainsod; Chaya Kalcheim; Joel K Yisraeli
Journal:  Development       Date:  2003-10-01       Impact factor: 6.868

5.  A new intrinsic fluorescent probe for proteins. Biosynthetic incorporation of 5-hydroxytryptophan into oncomodulin.

Authors:  C W Hogue; I Rasquinha; A G Szabo; J P MacManus
Journal:  FEBS Lett       Date:  1992-10-05       Impact factor: 4.124

Review 6.  Nonlinear magic: multiphoton microscopy in the biosciences.

Authors:  Warren R Zipfel; Rebecca M Williams; Watt W Webb
Journal:  Nat Biotechnol       Date:  2003-11       Impact factor: 54.908

7.  Mitochondrial citrate synthase is immobilized in vivo.

Authors:  P M Haggie; K M Brindle
Journal:  J Biol Chem       Date:  1999-02-12       Impact factor: 5.157

8.  A novel polypyrimidine tract-binding protein paralog expressed in smooth muscle cells.

Authors:  Clare Gooding; Paul Kemp; Christopher W J Smith
Journal:  J Biol Chem       Date:  2003-02-10       Impact factor: 5.157

9.  Spectral enhancement of proteins: biological incorporation and fluorescence characterization of 5-hydroxytryptophan in bacteriophage lambda cI repressor.

Authors:  J B Ross; D F Senear; E Waxman; B B Kombo; E Rusinova; Y T Huang; W R Laws; C A Hasselbacher
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-15       Impact factor: 11.205

10.  Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells.

Authors:  Zhiwen Zhang; Lital Alfonta; Feng Tian; Badry Bursulaya; Sean Uryu; David S King; Peter G Schultz
Journal:  Proc Natl Acad Sci U S A       Date:  2004-06-08       Impact factor: 11.205
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  2 in total

1.  Direct interaction of baculovirus capsid proteins VP39 and EXON0 with kinesin-1 in insect cells determined by fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy.

Authors:  John O Danquah; Stanley Botchway; Ananya Jeshtadi; Linda A King
Journal:  J Virol       Date:  2011-11-09       Impact factor: 5.103

2.  mTOR direct interactions with Rheb-GTPase and raptor: sub-cellular localization using fluorescence lifetime imaging.

Authors:  Rahul B Yadav; Pierre Burgos; Anthony W Parker; Valentina Iadevaia; Christopher G Proud; Rodger A Allen; James P O'Connell; Ananya Jeshtadi; Christopher D Stubbs; Stanley W Botchway
Journal:  BMC Cell Biol       Date:  2013-01-12       Impact factor: 4.241
  2 in total

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