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Literature DB >> 15937354

Detection of measles virus-specific interferon-gamma-secreting T-cells by ELISPOT.

Jenna E Ryan¹, Inna G Ovsyannikova, Gregory A Poland.

Abstract

The enzyme-linked immunospot (ELISPOT) assay is a highly sensitive tool used to measure the frequency of antigen-specific T-cells in vitro. Among its many applications, this assay is useful for the characterization of cellular immune responses after immunization against measles and other viral pathogens. A description of the measles virus-specific interferon (IFN)-gamma ELISPOT assay optimized in our laboratory is described in detail in this chapter. Procedures for the preparation of measles virus, infection of peripheral blood mononuclear cells with measles virus, and the IFN-gamma ELISPOT assay are also outlined. These methods can also be broadly adapted to measure activated T-cells to other viral pathogens and/or pathogen-derived peptides. Therefore, the ELISPOT assay can be used for the design, development, and evaluation of new vaccines.

Entities: Disease Species

Mesh：

Substances：
Interferon-gamma

Year: 2005 PMID： 15937354 DOI： 10.1385/1-59259-903-6:207

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

12 in total

1. Independence of measles-specific humoral and cellular immune responses to vaccination.

Authors: Robert M Jacobson; Inna G Ovsyannikova; Robert A Vierkant; V Shane Pankratz; Gregory A Poland
Journal: Hum Immunol Date: 2012-03-03 Impact factor: 2.850

2. Associations between demographic variables and multiple measles-specific innate and cell-mediated immune responses after measles vaccination.

Authors: Benjamin J Umlauf; Iana H Haralambieva; Inna G Ovsyannikova; Richard B Kennedy; V Shane Pankratz; Robert M Jacobson; Gregory A Poland
Journal: Viral Immunol Date: 2012-01-12 Impact factor: 2.257

3. Associations between polymorphisms in the antiviral TRIM genes and measles vaccine immunity.

Authors: Inna G Ovsyannikova; Iana H Haralambieva; Robert A Vierkant; Megan M O'Byrne; Gregory A Poland
Journal: Hum Immunol Date: 2013-02-13 Impact factor: 2.850

4. Replication of associations between cytokine and cytokine receptor single nucleotide polymorphisms and measles-specific adaptive immunophenotypic extremes.

Authors: Sarah J White; Iana H Haralambieva; Inna G Ovsyannikova; Robert A Vierkant; Megan M O'Byrne; Gregory A Poland
Journal: Hum Immunol Date: 2012-04-12 Impact factor: 2.850

5. Genetically defined race, but not sex, is associated with higher humoral and cellular immune responses to measles vaccination.

Authors: Emily A Voigt; Inna G Ovsyannikova; Iana H Haralambieva; Richard B Kennedy; Beth R Larrabee; Daniel J Schaid; Gregory A Poland
Journal: Vaccine Date: 2016-08-30 Impact factor: 3.641

10. 2'-5'-Oligoadenylate synthetase single-nucleotide polymorphisms and haplotypes are associated with variations in immune responses to rubella vaccine.

Authors: Iana H Haralambieva; Neelam Dhiman; Inna G Ovsyannikova; Robert A Vierkant; V Shane Pankratz; Robert M Jacobson; Gregory A Poland
Journal: Hum Immunol Date: 2010-01-31 Impact factor: 2.850

Detection of measles virus-specific interferon-gamma-secreting T-cells by ELISPOT.

1. Independence of measles-specific humoral and cellular immune responses to vaccination.

2. Associations between demographic variables and multiple measles-specific innate and cell-mediated immune responses after measles vaccination.

3. Associations between polymorphisms in the antiviral TRIM genes and measles vaccine immunity.

4. Replication of associations between cytokine and cytokine receptor single nucleotide polymorphisms and measles-specific adaptive immunophenotypic extremes.

5. Genetically defined race, but not sex, is associated with higher humoral and cellular immune responses to measles vaccination.

6. Effects of vitamin A and D receptor gene polymorphisms/haplotypes on immune responses to measles vaccine.

7. Correlation between rubella antibody levels and cytokine measures of cell-mediated immunity.

8. Effect of human leukocyte antigen homozygosity on rubella vaccine-induced humoral and cell-mediated immune responses.

9. Influence of host genetic variation on rubella-specific T cell cytokine responses following rubella vaccination.

10. 2'-5'-Oligoadenylate synthetase single-nucleotide polymorphisms and haplotypes are associated with variations in immune responses to rubella vaccine.