Literature DB >> 15933042

Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus.

N Jothikumar1, T L Cromeans, M D Sobsey, B H Robertson.   

Abstract

Primers and a TaqMan probe for the 5'-untranslated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provides an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.

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Year:  2005        PMID： 15933042      PMCID： PMC1151830          DOI： 10.1128/AEM.71.6.3359-3363.2005

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  28 in total

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3.  Hepatitis A in Latin America: a changing epidemiologic pattern.

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6.  Duration of viremia in hepatitis A virus infection.

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8.  Quantification and duration of viraemia during hepatitis A infection as determined by real-time RT-PCR.

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9.  A multistate, foodborne outbreak of hepatitis A. National Hepatitis A Investigation Team.

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  25 in total

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3.  Mineral Waste Containing High Levels of Iron from an Environmental Disaster (Bento Rodrigues, Mariana, Brazil) is Associated with Higher Titers of Enteric Viruses.

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4.  Hepatitis A Virus, Hepatitis E Virus, and Rotavirus in Foods of Animal Origin Traded at the Borders of Brazil, Argentina, and Uruguay.

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5.  A rapid single-tube protocol for HAV detection by nested real-time PCR.

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6.  Comparative analysis of viral concentration methods for detecting the HAV genome using real-time RT-PCR amplification.

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7.  Temperature-dependent survival of hepatitis A virus during storage of contaminated onions.

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8.  Microbial and chemical contamination during and after flooding in the Ohio River-Kentucky, 2011.

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9.  Multistate evaluation of an ultrafiltration-based procedure for simultaneous recovery of enteric microbes in 100-liter tap water samples.

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10.  Human and animal enteric virus in groundwater from deep wells, and recreational and network water.

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