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Literature DB >> 15931984

Using the flow cytometry to quantify the Giardia cysts and Cryptosporidium oocysts in water samples.

Bing-Mu Hsu¹, Nan-Min Wu, Hung-Der Jang, Feng-Cheng Shih, Min-Tao Wan, Chien-Min Kung.

Abstract

The flow cytometry (FC) has been used to detect Giardia cysts and Cryptosporidium oocysts quantitatively and instantaneously in this study. The experimental results showed that FC is potential to become a more precise method for the detection of Giardia and Cryptosporidium in water. This study also evaluated the staining efficiencies for three commercial antibodies. After staining Cryptosporidium oocysts with direct immunofluorescent antibodies in water samples, two populations were detected in the scatter-plots (FL1 versus SSC) of the FC. The Cryptosporidium oocysts and Giardia cysts are significantly separated from other particles while stained with direct immunofluorescent antibodies produced by Meridian Diagnostics and Waterborne Inc.

Entities: Chemical Disease Gene

Mesh：

Substances：
Water

Year: 2005 PMID： 15931984 DOI： 10.1007/s10661-005-1608-6

Source DB: PubMed Journal: Environ Monit Assess ISSN： 0167-6369 Impact factor: 2.513

5 in total

1. A simple method for evaluating Cryptosporidium-specific antibodies used in monitoring environmental water samples.

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Journal: Lett Appl Microbiol Date: 1997-11 Impact factor: 2.858

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Journal: Epidemiology Date: 1998-05 Impact factor: 4.822

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Authors: J F Perz; F K Ennever; S M Le Blancq
Journal: Am J Epidemiol Date: 1998-02-01 Impact factor: 4.897

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Journal: J Parasitol Date: 1995-06 Impact factor: 1.276

Review 5. Entamoeba histolytica and Giardia lamblia infections: current diagnostic strategies.

Authors: G C Cook
Journal: Parasite Date: 1995-06 Impact factor: 3.000

5 in total

7 in total

1. Chemical and microbial characteristics of municipal drinking water supply systems in the Canadian Arctic.

Authors: Kiley Daley; Lisbeth Truelstrup Hansen; Rob C Jamieson; Jenny L Hayward; Greg S Piorkowski; Wendy Krkosek; Graham A Gagnon; Heather Castleden; Kristen MacNeil; Joanna Poltarowicz; Emmalina Corriveau; Amy Jackson; Justine Lywood; Yannan Huang
Journal: Environ Sci Pollut Res Int Date: 2017-06-13 Impact factor: 4.223

2. Rapid detection and enumeration of Giardia lamblia cysts in water samples by immunomagnetic separation and flow cytometric analysis.

Authors: Hans-Anton Keserue; Hans Peter Füchslin; Thomas Egli
Journal: Appl Environ Microbiol Date: 2011-06-17 Impact factor: 4.792

Using the flow cytometry to quantify the Giardia cysts and Cryptosporidium oocysts in water samples.

1. A simple method for evaluating Cryptosporidium-specific antibodies used in monitoring environmental water samples.

2. An analysis of the Milwaukee cryptosporidiosis outbreak based on a dynamic model of the infection process.

3. Cryptosporidium in tap water: comparison of predicted risks with observed levels of disease.

4. A new method for evaluating experimental cryptosporidial parasite loads using immunofluorescent flow cytometry.

Review 5. Entamoeba histolytica and Giardia lamblia infections: current diagnostic strategies.

1. Chemical and microbial characteristics of municipal drinking water supply systems in the Canadian Arctic.

2. Rapid detection and enumeration of Giardia lamblia cysts in water samples by immunomagnetic separation and flow cytometric analysis.

Review 3. An overview of methods/techniques for the detection of Cryptosporidium in food samples.

4. IMS method performance analyses for Giardia in water under differing conditions.

5. Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samples.

6. Efficiency of chlorine and UV in the inactivation of Cryptosporidium and Giardia in wastewater.

7. Cryptosporidium as a testbed for single cell genome characterization of unicellular eukaryotes.