Xiao-Qing Li1, Lei Dong, Zhong-Hua Liu, Jin-Yan Luo. 1. Department of Gastroenterology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China. goodlxq@sina.com
Abstract
AIM: To investigate the mRNA expression of gamma-aminobutyric acid A (GABA(A)) receptor subunits alpha(1), beta(1), gamma(2) in different parts of the brain of rats with hepatic encephalopathy. METHODS: Twelve adult male Sprague-Dawley rats were randomly divided into two groups: (1) hepatic encephalopathy model group (n = 6), which was induced by intraperitoneal injection of thioacetamide (TAA, 350 mg/kg) for three consecutive days; (2) control group (n = 6), in which the rats were treated with same dose of normal saline solution. After the freeze slice of cerebrum was made, in situ hybridization was used to detect the mRNA of GABAA receptor subunits alpha(1), beta(1), and gamma(2) in rat cerebral cortex, basal nuclei, substantia nigra and hippocampi. Image data were collected and analyzed quantitatively by QWin550CW model image signal gather and analysis system. RESULTS: In rats with hepatic encephalopathy, mRNA expression levels of GABA(A) receptor subunits alpha(1), beta(1) increased significantly in basal nuclei, substantia nigra pars compacta, substantia nigra pars reticularis and hippocampi (144.7+/-15.67/184.14+/-4.41, 60.61+/-33.66/113.07+/-32.44, 87.71+/- 21.25/128.40+/-18.85, 122.34+/-5.56/161.60+/-4.56, 123.29+/-5.21/140.65+/-4.15, 123.40+/-4.42/140.09+/-4.52, 124.76+/-4.18/140.09+/-4.12, 141.62+/-15.09/182.80 +/-5.20, 69.13+/-30.74/134.21+/-43.76, 87.87+/-25.16/151.01+/-19.49, 122.14+/-6.30 /162.33+/-3.92, 122.81+/-5.09/137.19+/-7.12, 123.00+/-4.63/138.11+/-5.92, 125.75 +/-2.43/138.81+/-6.10, P<0.01), but did not change in the cerebral cortex compared to the control group. Similar changes were found in the mRNA expression levels of GABA(A) receptor subunit gamma(2), which increased significantly in basal nuclei, substantia nigra pars compacta, substantia nigra pars reticularis (136.81+/-26.41/167.97+/-16.23, 51.00+/-36.14/113.18+/-36.52, 86.35+/-20.30/ 126.90+/-19.74, P<0.01), CA1 of hippocampal (162.15+/-9.05/178.62+/-6.45, P<0.05), and no changes were found in the cerebral cortex and CA2, CA3, CA4 of hippocampi. CONCLUSION: In rats with hepatic encephalopathy, mRNA expression levels of GABA(A) receptor subunits alpha(1), beta(1), gamma(2) increase significantly in basal nuclei, substantia nigra and hippocampi, suggesting that the changes of mRNA expression levels in GABA(A) receptor subunits may contribute to the pathogenesis of hepatic encephalopathy.
AIM: To investigate the mRNA expression of gamma-aminobutyric acid A (GABA(A)) receptor subunits alpha(1), beta(1), gamma(2) in different parts of the brain of rats with hepatic encephalopathy. METHODS: Twelve adult male Sprague-Dawley rats were randomly divided into two groups: (1) hepatic encephalopathy model group (n = 6), which was induced by intraperitoneal injection of thioacetamide (TAA, 350 mg/kg) for three consecutive days; (2) control group (n = 6), in which the rats were treated with same dose of normal saline solution. After the freeze slice of cerebrum was made, in situ hybridization was used to detect the mRNA of GABAA receptor subunits alpha(1), beta(1), and gamma(2) in rat cerebral cortex, basal nuclei, substantia nigra and hippocampi. Image data were collected and analyzed quantitatively by QWin550CW model image signal gather and analysis system. RESULTS: In rats with hepatic encephalopathy, mRNA expression levels of GABA(A) receptor subunits alpha(1), beta(1) increased significantly in basal nuclei, substantia nigra pars compacta, substantia nigra pars reticularis and hippocampi (144.7+/-15.67/184.14+/-4.41, 60.61+/-33.66/113.07+/-32.44, 87.71+/- 21.25/128.40+/-18.85, 122.34+/-5.56/161.60+/-4.56, 123.29+/-5.21/140.65+/-4.15, 123.40+/-4.42/140.09+/-4.52, 124.76+/-4.18/140.09+/-4.12, 141.62+/-15.09/182.80 +/-5.20, 69.13+/-30.74/134.21+/-43.76, 87.87+/-25.16/151.01+/-19.49, 122.14+/-6.30 /162.33+/-3.92, 122.81+/-5.09/137.19+/-7.12, 123.00+/-4.63/138.11+/-5.92, 125.75 +/-2.43/138.81+/-6.10, P<0.01), but did not change in the cerebral cortex compared to the control group. Similar changes were found in the mRNA expression levels of GABA(A) receptor subunit gamma(2), which increased significantly in basal nuclei, substantia nigra pars compacta, substantia nigra pars reticularis (136.81+/-26.41/167.97+/-16.23, 51.00+/-36.14/113.18+/-36.52, 86.35+/-20.30/ 126.90+/-19.74, P<0.01), CA1 of hippocampal (162.15+/-9.05/178.62+/-6.45, P<0.05), and no changes were found in the cerebral cortex and CA2, CA3, CA4 of hippocampi. CONCLUSION: In rats with hepatic encephalopathy, mRNA expression levels of GABA(A) receptor subunits alpha(1), beta(1), gamma(2) increase significantly in basal nuclei, substantia nigra and hippocampi, suggesting that the changes of mRNA expression levels in GABA(A) receptor subunits may contribute to the pathogenesis of hepatic encephalopathy.
Authors: Samir Ahboucha; Paul Desjardins; Nicolas Chatauret; Gilles Pomier-Layrargues; Roger F Butterworth Journal: Neurochem Int Date: 2003-11 Impact factor: 3.921
Authors: Olga A Sergeeva; Olaf Kletke; Andrea Kragler; Anja Poppek; Wiebke Fleischer; Stephan R Schubring; Boris Görg; Helmut L Haas; Xin-Ran Zhu; Hermann Lübbert; Günter Gisselmann; Hanns Hatt Journal: J Biol Chem Date: 2010-05-28 Impact factor: 5.157