Search for germline alterations in CDKN2A/ARF and CDK4 of 42 Jewish melanoma families with or without neural system tumours.

To gain insight into the molecular mechanisms involved in the inherited predisposition to melanoma and associated neural system tumours, 42 Jewish, mainly Ashkenazi, melanoma families with or without neural system tumours were genotyped for germline point mutations and genomic deletions at the CDKN2A/ARF and CDK4 loci. CDKN2A/ARF deletion detection was performed using D9S1748, an intragenic microsatellite marker. Allele dosage at the p14ARF locus was analysed by quantitative real-time PCR employing a TaqMan probe that anneals specifically to exon 1beta of the p14ARF gene. For detecting point mutations, dHPLC and direct sequencing of the coding sequences of CDKN2A/ARF and CDK4 was used. No germline alterations in any of the tested genes were detected among the families under study. We conclude that in the majority of Ashkenazi Jewish families, the genes tested are unlikely to be implicated in the predisposition to melanoma and associated neural system tumours.

Familial cutaneous malignant melanoma is a genetically heterogeneous condition linked to chromosome 9p21 in many, but not all families (Hussussian ; Greene, 1999). To date, germline mutations in two high penetrance genes have been identified in some of these families, CDKN2A/ARF and CDK4. The CDKN2A/ARF gene encodes two distinct proteins, p16INK4 and p14ARF, the result of alternative splicing of exons 1α and 1β, respectively. The p16INK4 protein, which belongs to the INK4 family of cyclin-dependent kinase inhibitors, plays a key role in arresting cell cycle progression at the G1 phase by inhibiting cyclins CDK4 and CDK6 and subsequently blocking their ability to phosphorylate the retinoblastoma protein Rb (Chin ). The p14ARF protein is also involved in cell cycle regulation by interacting with different substrates in the p53 pathway (Pomerantz ), and by binding to MDM2, also in the Rb pathway with resultant cell cycle arrest in both G1 and G2 phases (Xiao ; Weber ; Momand ). In all, 20% of melanoma families were found to harbour genetic alterations at the CDKN2A/ARF gene (Goldstein, 2004). The other gene involved in familial predisposition to melanoma, CDK4 is a proto-oncogene that promotes cell cycle progression by phosphorylating the Rb protein. Germline mutations in CDK4 were detected in three melanoma families (Zuo ; Soufir ).

The familial clustering of both melanoma and neural system tumours (NST) was first reported in 1993 by Kaufman in a single family with eight family members over three generations who were diagnosed with cutaneous melanoma, cerebral astrocytoma or both. Azizi surveyed 904 melanoma Jewish-Israeli patients for the occurrence of NST in their family pedigrees. Melanoma-affected members within families, as well as first and second-degree relatives, were found to be at an increased risk for developing NST. A total of 15 families with a clustering of melanoma and a variety of NST were identified, and 10 patients with two primary tumours, melanoma and NST, primarily meningioma were described (Azizi ). Similar familial clustering of melanoma and NST was described in French (Bahuau ) and Finnish families (Paunu ). Recently, melanoma and NST association was confirmed by epidemiological and population-based studies in Scandinavia (O'Neill ; Hemminki ; Nielsen ). The familial clustering of melanoma and NST has been recognised and designated as the Melanoma and Neural System Tumour syndrome (MM-NST) (OMIM # 155755), and in a small subset of melanoma-NST kindreds germline mutations, mainly deletions affecting the CDKN2A/ARF gene and cosegregating with both tumours, were described.

In the present study, 42 Jewish, mainly Ashkenazi, melanoma families with (n=24) or without NST (n=18) were genotyped for germline sequence alterations in the CDKN2A/ARF and CDK4 genes. Mutational screening of 24 families with co-occurrence of melanoma and NST is the largest analysis reported thus far.

MATERIALS AND METHODS

Patients

Jewish families with a history of melanoma and NST were recruited to the study. The inclusion criteria (based on OMIM's definition) were a minimum of two cancers in the pedigree, one being melanoma and the other NST, or an individual harbouring both tumours.

Additional 18 Jewish melanoma families without NST, having at least two or more individuals with melanoma, or multiple melanomas in a single family member – as minimal inclusion criteria – were also included.

The families had been recruited between the years 1997 and 2003. The study had been approved by the Institutional Ethics Committee of the Sheba Medical Center, Israel. All participants signed a written informed consent prior to being enrolled in the study. Demographic details, including country of birth of the probands, their parents and grandparents, were collected using a self-response questionnaire. Classification to ethnic groups was done according to the country of birth of the grandparents on both the maternal and paternal sides, provided that one or both parents were either from the same origin, or Israeli-born. Families with both sets of grandparents from Eastern and Central European countries were classified as Ashkenazi. Families originating from Spain, North-Africa, Balkans, or Iraq, Iran, Yemen and Egypt were classified as Sephardic. Every effort has been made to confirm the correct cancer type for affected members based on pathology report, patients' medical charts, operation reports and death certificate. When these were not available, information regarding tumours that was obtained by history from several family members but not confirmed by a pathology report was designated as histological type not specified. A dermatological examination assessing skin phenotype, atypical mole syndrome (AMS) score (Newton Bishop ) and signs of dermato-heliosis was performed by one of three participating dermatologists and 10 ml of venous blood samples were withdrawn for DNA extraction.

Genetic alterations detection

DNA preparation

Genomic DNA was extracted from peripheral blood leucocytes using the Puregene® Genomic DNA Isolation Kit (Gentra Systems, Minneapolis MN, USA), using the manufacturer's recommended protocol.

Mutation analysis

For detecting CDKN2A/ARF and CDK4 gene coding region sequence alterations, exons 1α, 1β and 2 of CDKN2A/ARF and exon 2 of CDK4 were screened by dHPLC (denaturing high performance liquid chromatography), by using PCR and dHPLC analysis conditions previously described (Laud ). Briefly, PCR was carried out in a final volume of 20 μl containing 100 ng genomic DNA, 1 × HotStar Taq DNA Polymerase buffer with 1.5 mM MgCl2 (Qiagen), 4 pmoles of each primer, 1 UI HotStar Taq DNA Polymerase (Quiagen) and 2.5 mM dNTPs. For PCR amplification of each exon, a touch down protocol was used as follows: initial denaturation and HotStar Taq Polymerase activation at 95°C for 15 min; six cycles of 30 s at 95°C, 30 s at 66°C (the annealing temperature decreasing by 2°C at every two cycles), 30 s at 72°C; followed by 40 cycles of 30 s at 95°C, 30 s at 60°C and 30 s at 72°C. Heteroduplex analyses were carried out on an automated dHPLC instrument (WAVE, Transgenomic, CA, USA). DNA samples with known germline mutations at CDKN2A/ARF locus were used as positive controls.

Samples displaying abnormal profiles were subsequently bidirectionally sequenced using the BigDye™ Terminator sequencing kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions on an ABI Prism 377 instrument (Applied Biosystems, Foster City, CA, USA).

CDKN2A/ARF deletion detection

Since CDKN2A/ARF deletions were previously identified in melanoma-NST families (Bahuau , Randerson-Moor ), deletions were sought only in this subset of families (n=24). Deletion genotyping was performed using the D9S1748 microsatellite marker located adjacent to CDKN2A exon 1β. The PCR amplifications were carried out in a final volume of 25 μl, the reaction mix containing: 1 × HotStar Taq DNA polymerase buffer with 1.5 mM MgCl2 (Qiagen, Chatsworth, CA, USA), 1 UI HotStar Taq DNA polymerase (Qiagen), 4 pmoles of each primer and 0.2 mM dNTPs. Primer sequences are available through The Genome Database (http://www.gdb.org). The forward primer was fluorescently labeled with the 6-FAM at its 5′ extremity. The PCR products were loaded on a 6%/7 M urea denaturing polyacrylamide gel in an ABI Prism 377 (Applied Biosystems, Foster City, CA, USA) device along with the ROX 350 (Applied Biosystems, Foster City, CA, USA) internal marker standard. Genotypes were analysed using the GeneScan software (Applied Biosystems, Foster City, CA, USA). Since the homozygous status could possibly be due to the loss of an allele, homozygous samples were further analysed for allele dosage (Barrois ) at the p14ARF locus by quantitative real-time PCR using an ABI Prism 7700 instrument (Applied Biosystems, Foster City, CA, USA). A TaqMan probe that anneals specifically to the exon 1β of the p14ARF gene, marked with a fluorescent reporter dye (FAM) and a quencher dye (TAMRA), was used. By calculating the ratio initial copy number of p14ARF/initial copy number of GAPDH, we obtained the normalized gene dose. The PCR was performed in triplicate for each sample in a final volume of 50 μl, the reaction mix containing for the GAPDH gene 1 × TaqMan Universal Master Mix (Applied Biosystems, Foster City, CA, USA), 15 pmoles of each primer and probe and 25 ng DNA. For the exon 1β of CDKN2A, same quantities were used, with the exception of the TaqMan Universal Master Mix which was replaced by 1 × TaqMan PCR Core Reagent Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mM dNTPs, 5% glycerol, 5 mM MgCl2 and 1.25 UI AmpliTaqGold DNA polymerase (Applied Biosystems, Foster City, CA, USA). Amplification conditions were: 2 min at 50°C, 10 min at 95°C (20 min for p14ARF) followed by 40 cycles of 15 s at 95°C and 1 min at 60°C. We used as positive control the haploid cell line HL60 kindly provided by Juliette Moor and Julia Newton Bishop from Genetic Epidemiology Division, Cancer Research UK, St James's University Hospital, Leeds, UK (Randerson-Moor ).

RESULTS

Clinical features of the study participants

The study population included (a) 25 probands and 11 unaffected relatives from 24 families with pedigrees displaying cutaneous melanoma and NST and (b) 20 probands from 18 melanoma families without NST, among them 13 families with pedigrees containing two or more melanoma-affected individuals and five families containing individuals with multiple melanomas. Notably, the clinical features of two families (#107 and #121) have already been described earlier (Azizi ). Distribution by tumour type, number of tumours and family affiliation is presented in Tables 1 and 2 (melanoma-NST families) and 3 (familial melanoma).

Table 1

The distribution of melanoma-NST pedigrees according to number of tumours

No. of tumoursa	Family #	Total n (%)
NST × 2 and MM × 2	120	1 (0.5)
NST × 2 and MM × 1	116, 122, 113	3 (12.5)
MM × 2 and NST × 1	105, 109, 110 114, 118, 101	6 (25.0)
MM × 1 and NST × 1	112, 117, 119 102, 103, 104 106, 108, 111 115, 123, 124 107, 121	14 (58.0)
	Total	24 (100.0)

MM=melanoma, NST=neural system tumours.

Table 2

Distribution of melanoma-NST pedigrees by tumour type and family affiliation

	Proband					Affected relatives			Unaffected relatives
Family #	DNA#		MM (a)	NST (b)	Additional cancers	MM	NST (b)	Additional cancers
(n=24)	(n=25)	Gender	(Age at diagnosis)			(Age at diagnosis)			DNA#	Gender	Age
116	115	F	68	64 (c)	BCC (66)	—	Mother	Lung (uncle)	139	F	35
					SCC (69)		(47) (x)
120	218	M	51	51 (c)	BCC	—	—	Pleura (father)	—	—	—
	219	M	—	16 (b)	brother
122	221	M	51	51 (g)	—	—	Father (84) (x)	Colon (mother)	—	—	—
110	5	M	52	45 (f)	—	Sister (50)	—	—	4	M	15
									2	F	20
									3	M	21
105	88	F	58	68 (c)	—	—	—	—	—	—	—
			67
113	165	M	57	19 (x)	BCC	—	—	Lung (mother)	194	M	65
				25				Liver (cousin)
112	169	F	63	63 (e)	—	—	—	—	—	—	—
117	153	F	55	56 (c)	—	—	—	Breast (mother)	—	—	—
109	27	M	67		—	—	Brother (a)	—	67	M	45
	66	F	40		Daughter
114	73	F	22		—	Mother (48)	Mother (51,61) (e)	—	41	M	14
118	47	M	23		—	Mother (43)	Mother (51) (b)	—	—	—	—
101	154	M	32		BCC (37)	Mother (65)	Mother (68) (f)	—	185	F	45
119	—	—	—		—	Aunt	Sister (d)	Breast (sister, mother)	12	M	53
102	109	M	67		—	—	Grandson (24) (e)	—	133	F	45
103	6	M	40		—	—	Mother (71) (a)	Colon (mother) BCC/SCC Larynx (father) Liver (grand-mother)	—	—	—
104	13	F	67		Colon	—	Brother (a)	Colon (brother)	—	—	—
106	160	M	74		—	—	Father (68) (x)	—	166	M	75
108	131	M	67		—	—	Brother (57) (x)	—	—	—	—
111	113	F	66		—	—	Mother (x)	—	—	—	—
115	137	F	24		—	—	Grand-mother (86) (x)	—	—	—	—
123	222	F	56		—	—	Father (84) (x)	Colon (mother)	—	—	—
124	223	M	36		—	—	Cousin (30) (x)	Renal (father) Gastric (aunt)	—	—	—
107	83	F	43		—	—	Daughter (10) (h)	—	—	—	—
121	220	F	37		—	—	Grand-father (x)	Breast (mother)	—	—	—

MM=melanoma.

NST=neural system tumours, (a)=glioblastoma multiforme, (b)=oligodendroglioma, (c)=meningioma, (d)=glioma, (e)=neurilemmoma, (f)=malignant peripheral schwannoma, (g)=brain germinoma, (h)=medulloblastoma, (x)=NST, pathologic type unspecified.

Among the melanoma-NST pedigrees, in 10/24 (42%) families there were two melanoma or two NST cases, and in the others in this category, there were one melanoma and one NST in each family (Table 1). The male : female (M : F) ratio among the affected cases was 1. The melanoma and NST were diagnosed at the age range of 22–74 years and 10–86 years, respectively. Of the 24 families in this subgroup, 22 were of Ashkenazi origin, one out of 24 was Sephardic (#107), and one out of 24 heterogeneous (#121). Major phenotypic features of the patients, available in 13 out of 25 probands, were variable with no specific pattern. Additional cancers that were reported in this series included colon cancers in four families; breast cancer in three families; lung cancer in two families; liver cancer in two families; and renal, gastric, laryngeal, pleural and nonmelanoma skin cancer each in one family (Table 2). Examples of pedigrees showing melanoma families with NST are presented in Figure 1.

Figure 1

Representative pedigrees of six Jewish families with melanoma and neural system tumours (NST). Family codes of presented pedigrees (from left-to-right, top-to-bottom) – #110, #116, #118, #114, #109, #101. Striped squares and circles indicate male and female subjects with melanoma, respectively. Crossed symbols indicate individuals with neural system tumours and upper quarter filled symbols represent the presence of other tumours. Sample DNA code number and sequence alterations are indicated.

Among the 18 melanoma families without NST (Table 3), the M : F ratio among the melanoma patients was 1 : 2, and the age at diagnosis was in the range of 25–88 years. Of 18 families in this series, 17 were of Ashkenazi origin. Family #321 was of heterogeneous Romanian (Ashkenazi)/Turkish-Greek (Sephardic) origin. Major phenotypic features of the melanoma patients, not available for two out of 20 probands included dermato-heliosis and solar keratosis (15 out of 18), freckles (15 out of 18) and AMS ⩾2 (seven out of 18). Additional cancers that were reported in this series included nonmelanoma skin cancer in three families; prostate cancer in three; breast cancer in five; pancreatic cancer in one; transitional cell carcinoma in one and lymphoma in one. Examples of pedigrees showing melanoma families without NST are presented in Figure 2.

Table 3

Distribution of Pedigrees of melanoma families without neural system tumours, by tumour type and family affiliation

	Patient				Affected relatives
Family # (n=18)	DNA# (n=20)	Gender	MM (a)	Additional cancers	MM	Additional cancers
			(Age at diagnosis)		(Age at diagnosis)
302	104	F	74	—	Cousin (72)	—
303	103	M	49 (7 primary MM)	BCC SCC	Brother	—
305	100	F	74	BCC (75)	Nephew	—
309	112	F	52	—	—	Pancreas (father)
310	111	F	45 64	—	—	—
311	105	F	48	—	Sister	Breast (mother)
312	119	M	68	Prostate (68)	Mother (83) Brother (65) Daughter (19)	—
317	116	F	60	—	Grandmother	—
319	114	M	36	—	Cousin	—
321	15	F	25	—	Grandfather (61) Uncle	TCC (cousin) Breast (grandmother)
322	124	M	66	Prostate	—	Breast (mother)
			67	(67)
325	78	F	61 61	Breast (56)	—	—
	79	F	33	daughter
327	121	M	86	—	—	—
	135	F	88	sister
329	122	F	47 62	—	—	Lymphoma (mother)
330	140	F	67 71	—	Daughter	—
335	134	F	25	Breast	Uncle Grandfather	Breast (mother) BCC (father)
337	141	M	64 65	Prostate (56)	—	—
338	138	F	52 53	BCC (47)	Daughter (32)	—

MM=melanoma.

Figure 2

Representative pedigrees of four Jewish melanoma families without neural system tumours. Family codes of presented pedigrees (from left-to-right, top-to-bottom) – #312, #321, #325, #335. Striped squares and circles indicate male and female subjects with melanoma, respectively. Upper quarter filled symbols represent individuals with other tumours. Sample DNA code number and sequence alterations are indicated.

Mutational analyses of the CDKN2/ARF and the CDK4 genes for point mutations

Overall, nine samples displayed different chromatographic profiles. Sequence analyses revealed a G to A transition at position 442 leading to a missense mutation at codon 148 (Ala148Thr) in all nine patients: patients #5, #83 and #115, all unrelated, among the melanoma-NST families (Table 4); and patients #15, #111, #114, #116, #124, #134, all unrelated among the melanoma families without NST (Table 5).

Table 4

Mutation detection analysis in p16, p14 and CDK4 genes of melanoma-NST pedigrees (n=24)

Family # (n=24)	DNA # (n=36)	P16 sequencing analysis	P14 deletion analysis by D9S1748	Quantative TaqMan analysis of p14	p14 sequencing	CDK4 sequencing
101	154	WT	Hmz	2n	WT	WT
	185a	WT	Htz	ND	WT	WT
102	109	WT	Htz	ND	WT	WT
	133a	WT	Hmz	2n	WT	WT
103	6	WT	Hmz	2n	WT	WT
104	13	WT	Htz	ND	WT	WT
105	88	WT	Hmz	2n	WT	WT
106	160	WT	Htz	ND	WT	WT
	166a	WT	Htz	ND	WT	WT
107	83	Ala148Thr	Hmz	2n	WT	WT
108	131	WT	Htz	ND	WT	WT
109	27	WT	Htz	ND	WT	WT
	66	WT	Hmz	2n	WT	WT
	67a	WT	Hmz	2n	WT	WT
110	5	Ala148Thr	Hmz	2n	WT	WT
	4a	WT	Hmz	2n	WT	WT
	2a	WT	Hmz	2n	WT	WT
	3a	WT	Htz	ND	WT	WT
111	113	WT	Hmz	2n	WT	WT
112	169	WT	Htz	ND	WT	WT
113	165	WT	Hmz	2n	WT	WT
	194a	WT	Hmz	2n	WT	WT
114	73	WT	Hmz	2n	WT	WT
	41a	WT	Htz	ND	WT	WT
115	137	WT	Hmz	2n	WT	WT
116	115	Ala148Thr	Hmz	2n	WT	WT
	139a	WT	Hmz	2n	WT	WT
117	153	WT	Hmz	2n	WT	WT
118	47	WT	Htz	ND	WT	WT
119	12a	WT	Htz	ND	WT	WT
120	218	WT	ND	2n	WT	WT
	219	WT	ND	2n	WT	WT
121	220	WT	ND	2n	WT	WT
122	221	WT	ND	2n	WT	WT
123	222	WT	ND	2n	WT	WT
124	223	WT	ND	2n	WT	WT

Unaffected relatives.

Table 5

Mutation detection analysis in p16, p14 and CDK4 genes of pedigrees of melanoma families without neural system tumours (n=18)

Family # (n=18)	DNA # (n=20)	P16 sequencing analysis	P14 deletion analysis by D9S1748	Quantative TaqMan analysis of p14	p14 sequencing	CDK4 sequencing
302	104	WT	ND	ND	WT	WT
303	103	WT	ND	ND	WT	WT
305	100	WT	ND	ND	WT	WT
309	112	WT	ND	ND	WT	WT
310	111	Ala148Thr	ND	ND	WT	WT
311	105	WT	ND	ND	WT	WT
312	119	WT	ND	ND	WT	WT
317	116	Ala148Thr	ND	ND	WT	WT
319	114	Ala148Thr	ND	ND	WT	WT
321	15	Ala148Thr	ND	ND	WT	WT
322	124	Ala148Thr	ND	ND	WT	WT
325	78	WT	ND	ND	WT	WT
	79	WT	ND	ND	WT	WT
327	121	WT	ND	ND	WT	WT
	135	WT	ND	ND	WT	WT
329	122	WT	ND	ND	WT	WT
330	140	WT	ND	ND	WT	WT
335	134	Ala148Thr	ND	ND	WT	WT
337	141	WT	ND	ND	WT	WT
338	138	WT	ND	ND	WT	WT

Detection of CDKN2A/ARF gene deletions

A total of 30 individuals among the melanoma-NST families were genotyped using the D9S1748 microsatellite marker, located adjacent to exon 1β of CDKN2A/ARF gene on chromosome 9p21. A total of 12 samples displayed a heterozygous status, that is, two alleles without genomic deletion (Table 4); And 18 samples displaying homozygous profiles for this locus were selected for further analysis, since the homozygous status could indicate the loss of an allele by a large deletion encompassing exon 1β. Gene dosage for these samples, as well as six samples not analysed for the D9S1748 microsatellite marker, showed no deletions, therefore, all individuals presented two alleles (Table 4).

DISCUSSION

In the present study, no bona fide pathogenic germline alterations were identified in the CDKN2A/ARF and CDK4 loci among 42 Jewish, primarily Ashkenazi Israeli families, with a seemingly inherited predisposition to cutaneous melanoma, and in some, clustering of melanoma with NST, for deletions and point mutations in the CDKN2A/ARF and CDK4 loci. The only sequence variation identified in nine DNA samples was G to A transition at position 442 leading to a missense mutation at codon 148 (Ala148Thr). The Ala148Thr missense mutation is considered as a polymorphism based on several observations: it has been previously reported in individuals from the general, average risk, population in ethnically diverse groups: 8% of the Jewish population (Yakobson ), 4% of the population in Utah (Kamb ) and 5% in the UK population (Bertram ). Furthermore, this missense mutation did not segregate with the phenotype in familial melanoma (Hussussian ; Harland ), and is situated outside the critical four ankyrin repeat domains of p16, and thus does not appear to have any effect in vitro on binding to CDK4 (Ranade ; Lilischkis ; Harland ). Ala148Thr was further analysed in twin studies as a candidate low penetrance polymorphism enhancing the risk of melanoma by increasing AMS score (Zhu ). However, the rate of this polymorphism in families with atypical mole phenotype was similar to general population (Bertram ). Thus, Ala148Thr is considered a p16 polymorphism and not a pathogenic mutation.

Genetic mutations at the CDKN2A gene have been identified in 20% of melanoma families, most of these in exons 1α and 2 (Goldstein, 2004). Several studies also implicated the ARF gene as underlying melanoma predisposition. A 16 bp insertion in exon 1β, which affects the function of p14 but not p16, was described in a patient with multiple primary melanomas (Rizos ). A splice site mutation in exon 1β, which results in p14 haploinsufficiency, was also reported in two affected persons from melanoma kindred (Hewitt ). The negative mutation detection results in the present study among Jewish Ashkenazi melanoma families are not in line with the expected mutation rate, based on previously reported data in non-Jewish populations.

Among families with melanoma-NST association, the loss of function of CDKN2A/ARF can be a predisposing factor. Segregation analysis of two melanoma-NST French families showed hemizygous germline deletion that ablated CDKN2A/ARF gene (Bahuau ). Analysis of 11 families with two or more cases of glioma revealed a hemizygous germline deletion in CDKN2A in one family with both glioma and melanoma (Tachibana ). In another melanoma family with NST (mainly astrocytoma), deletion was found in the CDKN2A/ARF exon 1β. The deletion, leading to loss of ARF function, did not affect the coding region of p16 protein (Randerson-Moor ). Finally, a splice site substitution mutation trimming CDKN2A exon 2 and severely affecting both p16INK4A and p14ARF was described in a family with melanomas, neurofibromas and multiple dysplastic nevi (Petronzelli ). Constitutional CDKN2A locus alterations, somatic point mutations and deletions at CDKN2A were identified in NST (Ueki ; Bostrom ; Ghimenti ). Evidence was presented that deletion in ARF may be the underlying cause in the development of melanoma and NST (Randerson-Moor ).

Yet, the lack of mutations at the genes analysed in this series of melanoma families with NST is commensurate with data from other melanoma-NST families genotyped for mutations: in a family with melanoma and optic nerve glioma, no mutations were identified in the CDKN2A gene (Alao ). Analysis of Swedish patients with multiple primary melanomas and NST was negative for the CDKN2A founder mutation 113insArg, which usually explains all CDKN2A-associated familial melanoma in Sweden (Nielsen ).

It is unlikely that the familial clustering of melanoma and NST is due to chance, as both tumours are relatively rare cancers in Israel, with age standardised incidence rates of 7.7 and 8.4 per 105, respectively, in female subjects, and 7.6 and 10.3 per 105, respectively, in male subjects (Azizi ). All the melanoma families with NST in the present series withstand the OMIM criteria for the melanoma-NST syndrome. Yet, without identifying a mutation, cosegregating within the current series of families with both melanoma and NST, we cannot unequivocally determine the proportion of families that truly represent melanoma-NST syndrome. However, 10 families (42% of the series), having at least two melanoma or two NST probands, are strongly suggestive of an inherited predisposition for developing melanoma and NST.

Lack of germline mutations in the CDKN2A/ARF and CDK4 loci has been recently reported by Loo among 22 Ashkenazi Jewish families with an apparent inherited predisposition to melanoma. Taken together with the data reported herein, it appears that in over 60 Ashkenazi Jewish melanoma families, no germline alteration in CDKN2A/ARF and CDK4 loci underlie the apparent predisposition. One caveat to the present study that should be pointed out is that germline alterations in noncoding regions such as intronic and promoter sequences not screened in the present and in previous studies cannot be ruled out as contributing to familial melanoma.

In conclusion, in the majority of Ashkenazi Jewish families with an inherited predisposition to melanoma with or without NST, CDKN2A/ARF and CDK4 loci are unlikely to be implicated in the predisposition to melanoma and the associated neural system tumours.