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Literature DB >> 159275

Heparan sulfate at the surface of HeLa cells.

Abstract

HeLa cells, labeled with Na235SO4, release into the culture medium 35SO4 bound to plasma membrane vesicles next to 35SO4-glycoproteins and free 35SO4. Plasma membrane vesicles, experimentally produced by treatment with formaldehyde, contain 35SO4 and their surface can be stained with high iron diamine. Scanning of chromatograms of the trypsinate from labeled cells demonstrates radioactivity on the spot of heparan sulfate. It is concluded that HeLa cells synthesize heparan sulfate, which is incorporated at the plasma membrane and released by shedding of small vesicles.

Entities: Chemical

Mesh：

Substances：

Year: 1979 PMID： 159275 DOI： 10.1007/bf00644547

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

25 in total

1. Heparin releases heparan sulfate from the cell surface.

Authors: P M Kraemer
Journal: Biochem Biophys Res Commun Date: 1977-10-24 Impact factor: 3.575

2. Adhesion of malignant and nonmalignant cells to cultured embryonic substrates.

Authors: L de Ridder; M Mareel; L Vakaet
Journal: Cancer Res Date: 1975-11 Impact factor: 12.701

3. Cell recognition and adhesiveness: a possible biological role for the sulfated mucopolysaccharides.

Authors: C P Dietrich; L O Sampaio; O M Toledo; C M Cássaro
Journal: Biochem Biophys Res Commun Date: 1977-03-21 Impact factor: 3.575

4. Comparison of cell coat acid mucopolysaccharides of normal liver and various ascites hepatoma cells.

Authors: K Yamamoto; H Terayama
Journal: Cancer Res Date: 1973-10 Impact factor: 12.701

5. Differential diagnosis of mucopolysaccharidosis by means of thin-layer chromatography of urinary acidic glycosaminoglycans.

Authors: R Humbel; C Marchal; M Fall
Journal: Helv Paediatr Acta Date: 1969-12

Heparan sulfate at the surface of HeLa cells.

1. Heparin releases heparan sulfate from the cell surface.

2. Adhesion of malignant and nonmalignant cells to cultured embryonic substrates.

3. Cell recognition and adhesiveness: a possible biological role for the sulfated mucopolysaccharides.

4. Comparison of cell coat acid mucopolysaccharides of normal liver and various ascites hepatoma cells.

5. Differential diagnosis of mucopolysaccharidosis by means of thin-layer chromatography of urinary acidic glycosaminoglycans.

6. Intercellular glycosaminoglycans in normal and neoplastic tissues.

7. Search for collagen in substrate adhesion site of two murine cell lines.

8. Spontaneous shedding of plasma membrane fragments by human cells in vivo and in vitro.

9. Glycosaminoglycan synthesis by cultured human skin fibroblasts after transformation with simian virus 40.

10. Cell-cycle dependent desquamation of heparan sulfate from the cell surface.

Review 1. Invasion in vitro. Methods of analysis.