| Literature DB >> 15927276 |
D Bystricka1, O Lenz, I Mraz, L Piherova, S Kmoch, M Sip.
Abstract
Microarrays are one of the new emerging methods in plant virology currently being developed by various laboratories. In this study, a new approach is described on the detection of plant viruses using short synthetic single-stranded oligomers (40 nt) instead of PCR products as capture probes. A microchip detecting potato viruses, PVA, PVS, PVM, PVX, PVY and PLRV, in both single and mixed infections was developed and tested. The chip was also designed to distinguish between the main strains of PVY and PVS. Results of initial tests with PVY(NTN) and PVY(O) strains using several different probes for one virus are presented. Possibilities and advantages of the new oligonucleotide-based microarray approach for plant viral diagnosis are discussed.Entities:
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Year: 2005 PMID: 15927276 DOI: 10.1016/j.jviromet.2005.04.009
Source DB: PubMed Journal: J Virol Methods ISSN: 0166-0934 Impact factor: 2.014