BACKGROUND: The development and growth of human prostate cancer is mediated by many tumor cell-derived growth factors. Heparin affin regulatory peptide (HARP) seems to be involved in the progression of several tumors of diverse origin. In the present study, we sought to determine if HARP is implicated in human prostate cancer. METHODS: An antisense strategy for inhibition of HARP expression in the human prostate cancer cell line LNCaP was used to study the role of HARP on cancer cell growth, migration, and angiogenic potential in vitro and in vivo. RESULTS: Exogenous human recombinant HARP was mitogenic for LNCaP cells. By decreasing the expression of endogenous HARP, we found that HARP was essential for LNCaP cell migration, as well as anchorage-dependent and independent growth. Endothelial cell functions in vitro and blood vessel formation in vivo induced by LNCaP cells were also inhibited when HARP expression was diminished. CONCLUSIONS: HARP seems to act as an important regulator of diverse biological activities in human prostate cancer cells. Copyright 2005 Wiley-Liss, Inc.
BACKGROUND: The development and growth of humanprostate cancer is mediated by many tumor cell-derived growth factors. Heparin affin regulatory peptide (HARP) seems to be involved in the progression of several tumors of diverse origin. In the present study, we sought to determine if HARP is implicated in humanprostate cancer. METHODS: An antisense strategy for inhibition of HARP expression in the humanprostate cancer cell line LNCaP was used to study the role of HARP on cancer cell growth, migration, and angiogenic potential in vitro and in vivo. RESULTS: Exogenous human recombinant HARP was mitogenic for LNCaP cells. By decreasing the expression of endogenous HARP, we found that HARP was essential for LNCaP cell migration, as well as anchorage-dependent and independent growth. Endothelial cell functions in vitro and blood vessel formation in vivo induced by LNCaP cells were also inhibited when HARP expression was diminished. CONCLUSIONS:HARP seems to act as an important regulator of diverse biological activities in humanprostate cancer cells. Copyright 2005 Wiley-Liss, Inc.
Authors: Wei Huang; Jens C Eickhoff; Farideh Mehraein-Ghomi; Dawn R Church; George Wilding; Hirak S Basu Journal: Prostate Date: 2015-04-20 Impact factor: 4.104
Authors: Neville N C Tam; Carol Y Y Szeto; Johannes M Freudenberg; Amy N Fullenkamp; Mario Medvedovic; Shuk-Mei Ho Journal: Mol Endocrinol Date: 2010-09-22
Authors: Di Shen; Nataly P Podolnikova; Valentin P Yakubenko; Christopher L Ardell; Arnat Balabiyev; Tatiana P Ugarova; Xu Wang Journal: J Biol Chem Date: 2017-09-22 Impact factor: 5.157