Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Localization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts.

Literature DB >> 15923655

Localization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts.

Lisa A Mingle¹, Nataly N Okuhama, Jian Shi, Robert H Singer, John Condeelis, Gang Liu.

Abstract

The actin-related protein 2/3 (Arp2/3) complex is a crucial actin polymerization nucleator and is localized to the leading protrusions of migrating cells. However, how the multiprotein complex is targeted to the protrusions remains unknown. Here, we demonstrate that mRNAs for the seven subunits of the Arp2/3 complex are localized to the protrusions in fibroblasts, supporting a hypothesis that the Arp2/3 complex is targeted to its site of function by mRNA localization. Depletion of serum from culture medium inhibits Arp2/3-complex mRNA localization to the protrusion, whereas serum stimulation leads to significant mRNA localization within 30 minutes. The effect of serum suggests that Arp2/3-complex mRNA localization is a cellular response to extracellular stimuli. The localization of the Arp2/3 complex mRNAs is dependent on both actin filaments and microtubules, because disruption of either cytoskeletal system (with cytochalasin D and colchicine, respectively) inhibited the localization of all seven subunit mRNAs. In addition, myosin inhibitors significantly inhibit Arp2 mRNA localization in chicken embryo fibroblasts, suggesting a myosin motor dependent mechanism for Arp2/3-complex mRNA localization.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 2005 PMID： 15923655 PMCID： PMC1283079 DOI： 10.1242/jcs.02371

Source DB: PubMed Journal: J Cell Sci ISSN： 0021-9533 Impact factor: 5.285

43 in total

Localization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts.

1. Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor.

2. Single mRNA molecules demonstrate probabilistic movement in living mammalian cells.

3. RNA and RNA binding proteins participate in early stages of cell spreading through spreading initiation centers.

4. Selective inhibition of catalytic activity of smooth muscle myosin light chain kinase.

5. A Rho-dependent signaling pathway operating through myosin localizes beta-actin mRNA in fibroblasts.

6. Localization of actin messenger RNA during early ascidian development.

7. Use of oligodeoxynucleotide probes for quantitative in situ hybridization to actin mRNA.

8. A predominantly nuclear protein affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons.

9. 2,3-Butanedione monoxime (BDM) as a myosin inhibitor.

10. Two ZBP1 KH domains facilitate beta-actin mRNA localization, granule formation, and cytoskeletal attachment.

1. Organizing the oocyte: RNA localization meets phase separation.

2. How and why does Dia1 mRNA localize?

3. hnRNP Q regulates Cdc42-mediated neuronal morphogenesis.

4. Neuronal mRNAs travel singly into dendrites.

5. Localizing cellular housekeeping.

6. Regulation of protein levels in subcellular domains through mRNA transport and localized translation.

Review 7. Mechanisms and consequences of subcellular RNA localization across diverse cell types.

8. ZBP1 enhances cell polarity and reduces chemotaxis.

Review 9. Here, there, everywhere. mRNA localization in budding yeast.

10. Genome-wide screen reveals APC-associated RNAs enriched in cell protrusions.