Literature DB >> 15923356

Establishment of stable GH3 cell line expressing enhanced yellow fluorescein protein-growth hormone fusion protein.

Akira Matsuno1, Akiko Mizutani, Johbu Itoh, Susumu Takekoshi, Tadashi Nagashima, Hiroko Okinaga, Koji Takano, R Yoshiyuki Osamura.   

Abstract

To investigate, in real time, the transport and secretion of pituitary hormone, we have developed an experimental pituitary cell line, GH3 cell, which has secretory granules of growth hormone (GH) linked to enhanced yellow fluorescein protein (EYFP). This stable GH3 cell secretes secretory granules of GH linked to EYFP on stimulation by Ca2+ influx or Ca2 release from storage. This GH3 cell will be useful for the real-time visualization of the intracellular transport and secretion of GH.

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Year:  2005        PMID： 15923356     DOI： 10.1369/jhc.5B6708.2005

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  5 in total

1.  R-Ras regulates exocytosis by Rgl2/Rlf-mediated activation of RalA on endosomes.

Authors:  Akiyuki Takaya; Takahiro Kamio; Michitaka Masuda; Naoki Mochizuki; Hirofumi Sawa; Mami Sato; Kazuo Nagashima; Akiko Mizutani; Akira Matsuno; Etsuko Kiyokawa; Michiyuki Matsuda
Journal:  Mol Biol Cell       Date:  2007-03-07       Impact factor: 4.138

Review 2.  Functional molecular morphology of anterior pituitary cells, from hormone production to intracellular transport and secretion.

Authors:  Akira Matsuno; Akiko Mizutani; Hiroko Okinaga; Koji Takano; So Yamada; Shoko M Yamada; Hiroshi Nakaguchi; Katsumi Hoya; Mineko Murakami; Masato Takeuchi; Mutsumi Sugaya; Johbu Itoh; Susumu Takekoshi; R Yoshiyuki Osamura
Journal:  Med Mol Morphol       Date:  2011-06-30       Impact factor: 2.309

3.  SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5Kgamma Activity through Deacetylation of Specific Lysine Residues in Mammals.

Authors:  Sayaka Akieda-Asai; Nobuhiro Zaima; Koji Ikegami; Tomoaki Kahyo; Ikuko Yao; Takahiro Hatanaka; Shun-Ichiro Iemura; Rika Sugiyama; Takeaki Yokozeki; Yoshinobu Eishi; Morio Koike; Kyoji Ikeda; Takuya Chiba; Haruyoshi Yamaza; Isao Shimokawa; Si-Young Song; Akira Matsuno; Akiko Mizutani; Motoji Sawabe; Moses V Chao; Masashi Tanaka; Yasunori Kanaho; Tohru Natsume; Haruhiko Sugimura; Yukari Date; Michael W McBurney; Leonard Guarente; Mitsutoshi Setou
Journal:  PLoS One       Date:  2010-07-23       Impact factor: 3.240

Review 4.  Molecular morphology of pituitary cells, from conventional immunohistochemistry to fluorescein imaging.

Authors:  Akira Matsuno; Akiko Mizutani; Hiroko Okinaga; Koji Takano; So Yamada; Shoko M Yamada; Hiroshi Nakaguchi; Katsumi Hoya; Mineko Murakami; Masato Takeuchi; Mutsumi Sugaya; Johbu Itoh; Susumu Takekoshi; R Yoshiyuki Osamura
Journal:  Molecules       Date:  2011-04-29       Impact factor: 4.411

5.  Direct visualization of glucagon-like peptide-1 secretion by fluorescent fusion proteins.

Authors:  Atsushi Tsuzuki; Yoichiro Fujioka; Aiko Yoshida; Sayaka Kashiwagi; Maho Amano; Tohru Hira; Akinobu Nakamura; Hideaki Miyoshi; Tatsuya Atsumi; Yusuke Ohba
Journal:  J Diabetes Investig       Date:  2022-04-18       Impact factor: 3.681
  5 in total

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