Literature DB >> 1592122

Crystallization and preliminary X-ray analysis of recombinant human transforming growth factor beta 2.

M P Schlunegger1, N Cerletti, D A Cox, G K McMaster, A Schmitz, M G Grütter.   

Abstract

Recombinant human transforming growth factor beta 2 (TGF-beta 2) was cloned and expressed in E. coli. The protein was isolated from inclusion bodies, renatured and purified to a single component as judged by reversed-phase HPLC. The recombinant TGF-beta 2 was shown to have a biological activity equal to that of native TGF-beta 2 in a fibroblast migration assay. Pure, active recombinant TGF-beta 2 has been crystallized from polyethylene glycol 400. The trigonal crystals of spacegroup P3(1)21 or P3(2)21 have unit cell dimensions of a=b=60.6 A, c=75.2 A and diffract beyond 2.0 A.

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Year:  1992        PMID： 1592122     DOI： 10.1016/0014-5793(92)80484-x

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  7 in total

1.  Ectopic induction of tendon and ligament in rats by growth and differentiation factors 5, 6, and 7, members of the TGF-beta gene family.

Authors:  N M Wolfman; G Hattersley; K Cox; A J Celeste; R Nelson; N Yamaji; J L Dube; E DiBlasio-Smith; J Nove; J J Song; J M Wozney; V Rosen
Journal:  J Clin Invest       Date:  1997-07-15       Impact factor: 14.808

Review 2.  The role of growth/differentiation factor 5 (GDF5) in the induction and survival of midbrain dopaminergic neurones: relevance to Parkinson's disease treatment.

Authors:  Aideen M Sullivan; Gerard W O'Keeffe
Journal:  J Anat       Date:  2005-09       Impact factor: 2.610

3.  Crystallization and preliminary X-ray diffraction analysis of human growth and differentiation factor 5 (GDF-5).

Authors:  Thomas D Mueller; Maike Gottermeier; Walter Sebald; Joachim Nickel
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2004-12-24

4.  Oral mucosal permeability and stability of transforming growth factor beta-3 in vitro.

Authors:  C A Squier; M J Kremer; A Bruskin; A Rose; J D Haley
Journal:  Pharm Res       Date:  1999-10       Impact factor: 4.200

5.  Pretreatment with transforming growth factor beta-3 protects small intestinal stem cells against radiation damage in vivo.

Authors:  C S Potten; D Booth; J D Haley
Journal:  Br J Cancer       Date:  1997       Impact factor: 7.640

6.  Recombinant production, purification, crystallization, and structure analysis of human transforming growth factor β2 in a new conformation.

Authors:  Laura Del Amo-Maestro; Laura Marino-Puertas; Theodoros Goulas; F Xavier Gomis-Rüth
Journal:  Sci Rep       Date:  2019-06-17       Impact factor: 4.379

7.  Structure-based engineering to restore high affinity binding of an isoform-selective anti-TGFβ1 antibody.

Authors:  Dana M Lord; Julie J Bird; Denise M Honey; Annie Best; Anna Park; Ronnie R Wei; Huawei Qiu
Journal:  MAbs       Date:  2018-02-01       Impact factor: 5.857
  7 in total

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