Literature DB >> 1592049

A rapid polymerase chain reaction method for identifying fixed specimens.

P Gill1, C P Kimpton, K Sullivan.   

Abstract

DNA profiling analysis of preserved tissue samples was achieved by using an extraction method which incorporated the use of a chelating resin "Chelex" to chelate inhibitory polyvalent metal ions. An automated sequencer was used to estimate sizes of low molecular weight trimeric and tetrameric microsatellites labelled with fluorescent dyes. The entire analysis can be completed within 48h. Probabilities of chance association using two microsatellites were calculated as ranging between 10(-2) and 10(-4). The methods described have considerable potential for use in routine genetic analysis of preserved samples.

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Year:  1992        PMID： 1592049     DOI： 10.1002/elps.1150130135

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  9 in total

1.  Population genetic study from the Zagreb area using 3 STR systems.

Authors:  M Kubat; P Wiegand; B Brinkmann
Journal:  Int J Legal Med       Date:  1995       Impact factor: 2.686

2.  Spanish population data on 7 tetrameric short tandem repeat loci.

Authors:  P Martin; A Alonso; B Budowle; C Albarrán; O García; M Sancho
Journal:  Int J Legal Med       Date:  1995       Impact factor: 2.686

3.  Analysis of 6 VNTR loci by 'multiplex' PCR and automated fluorescent detection.

Authors:  G Tully; K M Sullivan; P Gill
Journal:  Hum Genet       Date:  1993-12       Impact factor: 4.132

4.  Frequency profiles of 3 STRs in a Turkish population.

Authors:  B Alper; P Wiegand; B Brinkmann
Journal:  Int J Legal Med       Date:  1995       Impact factor: 2.686

5.  Evaluation of an automated DNA profiling system employing multiplex amplification of four tetrameric STR loci.

Authors:  C Kimpton; D Fisher; S Watson; M Adams; A Urquhart; J Lygo; P Gill
Journal:  Int J Legal Med       Date:  1994       Impact factor: 2.686

6.  Swiss population data on three tetrameric short tandem repeat loci--VWA, HUMTHO1, and F13A1--derived using multiplex PCR and laser fluorescence detection.

Authors:  M N Hochmeister; J M Jung; B Budowle; U V Borer; R Dirnhofer
Journal:  Int J Legal Med       Date:  1994       Impact factor: 2.686

7.  Simultaneous analysis of six microsatellite markers in Atlantic cod ( Gadus morhua): a novel multiplex assay system for use in selective breeding studies.

Authors:  Madjid Delghandi; Atle Mortensen; Jon-Ivar Westgaard
Journal:  Mar Biotechnol (NY)       Date:  2003 Mar-Apr       Impact factor: 3.619

8.  Rapid techniques for DNA extraction from routinely processed archival tissue for use in PCR.

Authors:  R Sepp; I Szabó; H Uda; H Sakamoto
Journal:  J Clin Pathol       Date:  1994-04       Impact factor: 3.411

9.  Genetic analysis of two STR loci (VWA and TPOX) in the Iranian province of Khuzestan.

Authors:  Ali Mohammad Foroughmand; Maryam Jari; Seyed Reza Kazeminezhad; Arezu Abdollahi; Leila Ahmadi; Maryam Heidari
Journal:  Iran J Basic Med Sci       Date:  2014-08       Impact factor: 2.699
  9 in total

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