Literature DB >> 15919908

Mutations in the NS1 protein of swine influenza virus impair anti-interferon activity and confer attenuation in pigs.

Alicia Solórzano1, Richard J Webby, Kelly M Lager, Bruce H Janke, Adolfo García-Sastre, Jürgen A Richt.   

Abstract

It has been shown previously that the nonstructural protein NS1 of influenza virus is an alpha/beta interferon (IFN-alpha/beta) antagonist, both in vitro and in experimental animal model systems. However, evidence of this function in a natural host has not yet been obtained. Here we investigated the role of the NS1 protein in the virulence of a swine influenza virus (SIV) isolate in pigs by using reverse genetics. The virulent wild-type A/Swine/Texas/4199-2/98 (TX/98) virus and various mutants encoding carboxy-truncated NS1 proteins were rescued. Growth properties of TX/98 viruses with mutated NS1, induction of IFN in tissue culture, and virulence-attenuation in pigs were analyzed and compared to those of the recombinant wild-type TX/98 virus. Our results indicate that deletions in the NS1 protein decrease the ability of the TX/98 virus to prevent IFN-alpha/beta synthesis in pig cells. Moreover, all NS1 mutant viruses were attenuated in pigs, and this correlated with the amount of IFN-alpha/beta induced in vitro. These data suggest that the NS1 protein of SIV is a virulence factor. Due to their attenuation, NS1-mutated swine influenza viruses might have a great potential as live attenuated vaccine candidates against SIV infections of pigs.

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Year:  2005        PMID： 15919908      PMCID： PMC1143661          DOI： 10.1128/JVI.79.12.7535-7543.2005

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  54 in total

1.  Characterization of influenza virus NS1 protein by using a novel helper-virus-free reverse genetic system.

Authors:  M Enami; K Enami
Journal:  J Virol       Date:  2000-06       Impact factor: 5.103

2.  The prevalence of influenza viruses in swine and the antigenic and genetic relatedness of influenza viruses from man and swine.

Authors:  V S Hinshaw; W J Bean; R G Webster; B C Easterday
Journal:  Virology       Date:  1978-01       Impact factor: 3.616

3.  Genetic reassortment of avian, swine, and human influenza A viruses in American pigs.

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Journal:  J Virol       Date:  1999-10       Impact factor: 5.103

4.  Development of the interferon system. I. In chicken cells development in ovo continues on time in vitro.

Authors:  M J Sekellick; W J Biggers; P I Marcus
Journal:  In Vitro Cell Dev Biol       Date:  1990-10

5.  Influenza viral infection of swine in the United States 1988-1989.

Authors:  T M Chambers; V S Hinshaw; Y Kawaoka; B C Easterday; R G Webster
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

6.  Interferon induction by viruses. XIV. Development of interferon inducibility and its inhibition in chick embryo cells "aged" in vitro.

Authors:  M J Sekellick; P I Marcus
Journal:  J Interferon Res       Date:  1985

7.  Animals in the influenza world.

Authors:  B C Easterday
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1980-02-25       Impact factor: 6.237

8.  Defective RNA replication and late gene expression in temperature-sensitive influenza viruses expressing deleted forms of the NS1 protein.

Authors:  Ana M Falcón; Rosa M Marión; Thomas Zürcher; Paulino Gómez; Agustín Portela; Amelia Nieto; Juan Ortín
Journal:  J Virol       Date:  2004-04       Impact factor: 5.103

9.  The NS1 gene of H5N1 influenza viruses circumvents the host anti-viral cytokine responses.

Authors:  Sang Heui Seo; Erich Hoffmann; Robert G Webster
Journal:  Virus Res       Date:  2004-07       Impact factor: 3.303

10.  Multiple lineages of antigenically and genetically diverse influenza A virus co-circulate in the United States swine population.

Authors:  R J Webby; K Rossow; G Erickson; Y Sims; R Webster
Journal:  Virus Res       Date:  2004-07       Impact factor: 3.303
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  133 in total

1.  Glycine 184 in nonstructural protein NS1 determines the virulence of influenza A virus strain PR8 without affecting the host interferon response.

Authors:  Sabine Steidle; Luis Martínez-Sobrido; Markus Mordstein; Stefan Lienenklaus; Adolfo García-Sastre; Peter Stäheli; Georg Kochs
Journal:  J Virol       Date:  2010-10-06       Impact factor: 5.103

2.  Intracellular distribution of NS1 correlates with the infectivity and interferon antagonism of an avian influenza virus (H7N1).

Authors:  Bjoern Keiner; Benjamin Maenz; Ralf Wagner; Giovanni Cattoli; Ilaria Capua; Hans-Dieter Klenk
Journal:  J Virol       Date:  2010-09-15       Impact factor: 5.103

3.  Pathogenicity of swine influenza viruses possessing an avian or swine-origin PB2 polymerase gene evaluated in mouse and pig models.

Authors:  Wenjun Ma; Kelly M Lager; Xi Li; Bruce H Janke; Derek A Mosier; Laura E Painter; Eva S Ulery; Jingqun Ma; Porntippa Lekcharoensuk; Richard J Webby; Jürgen A Richt
Journal:  Virology       Date:  2010-11-11       Impact factor: 3.616

4.  The cysteine protease domain of porcine reproductive and respiratory syndrome virus nonstructural protein 2 possesses deubiquitinating and interferon antagonism functions.

Authors:  Zhi Sun; Zhenhai Chen; Steven R Lawson; Ying Fang
Journal:  J Virol       Date:  2010-05-26       Impact factor: 5.103

5.  Functional Evolution of Influenza Virus NS1 Protein in Currently Circulating Human 2009 Pandemic H1N1 Viruses.

Authors:  Amelia M Clark; Aitor Nogales; Luis Martinez-Sobrido; David J Topham; Marta L DeDiego
Journal:  J Virol       Date:  2017-08-10       Impact factor: 5.103

6.  Functional genomic and serological analysis of the protective immune response resulting from vaccination of macaques with an NS1-truncated influenza virus.

Authors:  C R Baskin; H Bielefeldt-Ohmann; A García-Sastre; T M Tumpey; N Van Hoeven; V S Carter; M J Thomas; S Proll; A Solórzano; R Billharz; J L Fornek; S Thomas; C-H Chen; E A Clark; Kaja Murali-Krishna; M G Katze
Journal:  J Virol       Date:  2007-08-22       Impact factor: 5.103

7.  Live attenuated influenza viruses containing NS1 truncations as vaccine candidates against H5N1 highly pathogenic avian influenza.

Authors:  John Steel; Anice C Lowen; Lindomar Pena; Matthew Angel; Alicia Solórzano; Randy Albrecht; Daniel R Perez; Adolfo García-Sastre; Peter Palese
Journal:  J Virol       Date:  2008-12-10       Impact factor: 5.103

8.  Interferon Inducer IFI35 regulates RIG-I-mediated innate antiviral response through mutual antagonism with Influenza protein NS1.

Authors:  Hui Yang; Wendy Winkler; Xiaopeng Wu
Journal:  J Virol       Date:  2021-03-10       Impact factor: 5.103

9.  The NS segment of an H5N1 highly pathogenic avian influenza virus (HPAIV) is sufficient to alter replication efficiency, cell tropism, and host range of an H7N1 HPAIV.

Authors:  Wenjun Ma; Dominique Brenner; Zhongfang Wang; Bianca Dauber; Christina Ehrhardt; Katrin Högner; Susanne Herold; Stephan Ludwig; Thorsten Wolff; Kangzhen Yu; Jürgen A Richt; Oliver Planz; Stephan Pleschka
Journal:  J Virol       Date:  2009-12-09       Impact factor: 5.103

10.  Contribution of double-stranded RNA and CPSF30 binding domains of influenza virus NS1 to the inhibition of type I interferon production and activation of human dendritic cells.

Authors:  Irene Ramos; Elena Carnero; Dabeiba Bernal-Rubio; Christopher W Seibert; Liset Westera; Adolfo García-Sastre; Ana Fernandez-Sesma
Journal:  J Virol       Date:  2012-12-19       Impact factor: 5.103
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