| Literature DB >> 15919141 |
Winfried G J Degen1, Hanneke I van Zuilekom, Nicolette C Scholtes, Nancy van Daal, Virgil E J C Schijns.
Abstract
Mammalian interleukin-18 (IL-18) is one of the pro-inflammatory cytokines involved in innate immune responses to microbial infection preceding the induction of both cellular and humoral immune responses. We assessed the potential of Escherichia coli-expressed His-tag purified recombinant chicken IL-18 (rHis-ChIL-18) as a potentiator of vaccine-induced immune responses in 3 week-old SPF chickens and compared it with several commonly used traditional immunostimulating adjuvants. We found that rHis-ChIL-18 significantly enhanced antibody responses to Clostridium perfringens alpha-toxoid and Newcastle disease virus (NDV) antigens, comparable to the Al(OH)3-gel, Miglyol and chitosan adjuvant. However, antibody responses were lower than water-in-oil (w/o) emulsions and combinations of rHis-ChIL-18 with Al(OH)3-gel, Miglyol or chitosan. Improved HI-titers for infectious bronchitis virus (IBV) were not noted. We conclude that rHis-ChIL-18, when delivered in the proper context, is a novel and safe immunostimulator in chickens.Entities:
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Year: 2005 PMID： 15919141 DOI： 10.1016/j.vaccine.2005.04.003
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641