| Literature DB >> 15916053 |
Yoshinori Hirose1, Masashi Kirinoki, Yuichi Chigusa, Hajime Matsuda.
Abstract
A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits was compared with a conventional ELISA. This whole-blood ELISA has advantages. The volume of crude egg antigens, whole blood samples, and conjugates was only 0.05 ml. The incubation time was shortened to 5 minutes. Wells were washed three to five times with PBS-Tween after each procedure. Optical density values were measured in 10 minutes after transfer of 0.1 ml of substrate. Constant temperature was not necessary. The entire procedure took only 20-30 minutes.Entities:
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Year: 2005 PMID： 15916053
Source DB: PubMed Journal: Southeast Asian J Trop Med Public Health ISSN： 0125-1562 Impact factor: 0.267