BACKGROUND: The hantavirus cardiopulmonary syndrome (HCPS) was first recognized in 1993 after a cluster of acute respiratory distress syndrome deaths in the southwestern of the United States. The major causative agent of HCPS in North America is the Sin Nombre virus (SNV) carried by the deer mouse Peromyscus maniculatus. The first HCPS case imported to Europe was reported in 2002. OBJECTIVES: The objective of the study was to develop and evaluate ELISA and Western blot tests for the serological detection of human infections caused by SNV including those imported to Europe. STUDY DESIGN: A polyhistidine (His)-tagged recombinant nucleocapsid (rN) protein of SNV was expressed in Saccharomyces cerevisiae and purified by nickel chelation chromatography. On the basis of the purified SNV rN protein mu-capture and indirect IgM and IgG ELISAs and an IgG Western blot were developed. The evaluation of the tests was performed using a negative serum panel and a blinded serum panel from the US containing acute-phase sera from HCPS patients. RESULTS: Based upon the results obtained using a panel of negative control sera the specificity for SNV mu-capture and indirect IgM and IgG ELISAs were found to be 100%. All 33 sera from SNV-infected HCPS patients included in the blinded panel were detected by the SNV mu-capture and indirect IgM ELISAs. Twenty-nine out of the 33 SNV-IgM positive sera reacted also in the SNV-IgG ELISA. An SNV-IgG Western blot confirmed the data of the SNV-IgG ELISA. Although the majority of anti-SNV positive sera cross-reacted with rN proteins of Puumala virus and Dobrava virus, the lacking reactivity of a few sera with these heterologous rN antigens in the corresponding IgM and IgG ELISAs demonstrates the value of virus-specific test formats for acute-phase sera. CONCLUSIONS: The novel SNV ELISA and Western blot tests represent a useful tool for the serological detection of SNV infections.
BACKGROUND: The hantavirus cardiopulmonary syndrome (HCPS) was first recognized in 1993 after a cluster of acute respiratory distress syndrome deaths in the southwestern of the United States. The major causative agent of HCPS in North America is the Sin Nombre virus (SNV) carried by the deer mousePeromyscus maniculatus. The first HCPS case imported to Europe was reported in 2002. OBJECTIVES: The objective of the study was to develop and evaluate ELISA and Western blot tests for the serological detection of humaninfections caused by SNV including those imported to Europe. STUDY DESIGN: A polyhistidine (His)-tagged recombinant nucleocapsid (rN) protein of SNV was expressed in Saccharomyces cerevisiae and purified by nickel chelation chromatography. On the basis of the purified SNV rN protein mu-capture and indirect IgM and IgG ELISAs and an IgG Western blot were developed. The evaluation of the tests was performed using a negative serum panel and a blinded serum panel from the US containing acute-phase sera from HCPS patients. RESULTS: Based upon the results obtained using a panel of negative control sera the specificity for SNV mu-capture and indirect IgM and IgG ELISAs were found to be 100%. All 33 sera from SNV-infected HCPSpatients included in the blinded panel were detected by the SNV mu-capture and indirect IgM ELISAs. Twenty-nine out of the 33 SNV-IgM positive sera reacted also in the SNV-IgG ELISA. An SNV-IgG Western blot confirmed the data of the SNV-IgG ELISA. Although the majority of anti-SNV positive sera cross-reacted with rN proteins of Puumala virus and Dobrava virus, the lacking reactivity of a few sera with these heterologous rN antigens in the corresponding IgM and IgG ELISAs demonstrates the value of virus-specific test formats for acute-phase sera. CONCLUSIONS: The novel SNV ELISA and Western blot tests represent a useful tool for the serological detection of SNV infections.
Authors: Marc Mertens; Jörg Hofmann; Rasa Petraityte-Burneikiene; Mario Ziller; Kestutis Sasnauskas; Robert Friedrich; Olaf Niederstrasser; Detlev H Krüger; Martin H Groschup; Eckhardt Petri; Sandra Werdermann; Rainer G Ulrich Journal: Med Microbiol Immunol Date: 2011-05-25 Impact factor: 3.402
Authors: Marc Mertens; Eveline Kindler; Petra Emmerich; Jutta Esser; Christiane Wagner-Wiening; Roman Wölfel; Rasa Petraityte-Burneikiene; Jonas Schmidt-Chanasit; Aurelija Zvirbliene; Martin H Groschup; Gerhard Dobler; Martin Pfeffer; Gerald Heckel; Rainer G Ulrich; Sandra S Essbauer Journal: Virus Genes Date: 2011-05-20 Impact factor: 2.332
Authors: S Essbauer; J Schmidt; F J Conraths; R Friedrich; J Koch; W Hautmann; M Pfeffer; R Wölfel; J Finke; G Dobler; R Ulrich Journal: Epidemiol Infect Date: 2006-05-02 Impact factor: 2.451
Authors: Marc Mertens; Roman Wölfel; Katrin Ullrich; Kumiko Yoshimatsu; Jana Blumhardt; Ina Römer; Jutta Esser; Jonas Schmidt-Chanasit; Martin H Groschup; Gerhard Dobler; Sandra S Essbauer; Rainer G Ulrich Journal: Med Microbiol Immunol Date: 2009-01-16 Impact factor: 3.402
Authors: Sabine Lederer; Erik Lattwein; Merle Hanke; Karen Sonnenberg; Winfried Stoecker; Åke Lundkvist; Antti Vaheri; Olli Vapalahti; Paul K S Chan; Heinz Feldmann; Daryl Dick; Jonas Schmidt-Chanasit; Paula Padula; Pablo A Vial; Raluca Panculescu-Gatej; Cornelia Ceianu; Paul Heyman; Tatjana Avšič-Županc; Matthias Niedrig Journal: PLoS Negl Trop Dis Date: 2013-04-04
Authors: Dennis J Cleri; Anthony J Ricketti; Richard B Porwancher; Luz S Ramos-Bonner; John R Vernaleo Journal: Infect Dis Clin North Am Date: 2006-06 Impact factor: 5.982