BACKGROUND: The detection of the IgM antibody for the dengue virus in serum by ELISA has become one of the most important and useful methods for diagnosis of dengue using a single acute-phase serum sample. Currently, this system is an invaluable tool for the surveillance of dengue fever (DF) and dengue hemorrhagic fever (DHF). The usefulness of other serological markers such as IgA and IgE have been less studied. OBJECTIVE: To study the IgM, IgA and IgE specific antibody response in dengue 3 infected patients with different clinical picture and type of infection. STUDY DESIGN: One hundred and twenty-seven serum samples collected on days 5-7 at the onset of fever from clinically and serologically confirmed dengue cases were studied. Forty-two were classified as primary dengue fever cases, 48 as secondary dengue fever cases and 37 as secondary dengue hemorrhagic fever cases. All samples were tested by capture ELISA in order to detect dengue IgM, IgA and IgE antibodies. RESULTS AND CONCLUSIONS: In this study, significant differences were observed in the IgM, IgA and IgE response between the study groups. High IgA and IgE OD ratios in secondary dengue cases were found. The usefulness of serotype specific IgM antibody detection is also analyzed and discussed. A priority for future dengue research in terms of protection, recovery of infection and immunopathogenesis is to elucidate the role of these immunoglobulins. The cross reactivity response to IgM between dengue virus serotypes in primary and secondary cases should also be more studied.
BACKGROUND: The detection of the IgM antibody for the dengue virus in serum by ELISA has become one of the most important and useful methods for diagnosis of dengue using a single acute-phase serum sample. Currently, this system is an invaluable tool for the surveillance of dengue fever (DF) and dengue hemorrhagic fever (DHF). The usefulness of other serological markers such as IgA and IgE have been less studied. OBJECTIVE: To study the IgM, IgA and IgE specific antibody response in dengue 3 infectedpatients with different clinical picture and type of infection. STUDY DESIGN: One hundred and twenty-seven serum samples collected on days 5-7 at the onset of fever from clinically and serologically confirmed dengue cases were studied. Forty-two were classified as primary dengue fever cases, 48 as secondary dengue fever cases and 37 as secondary dengue hemorrhagic fever cases. All samples were tested by capture ELISA in order to detect dengue IgM, IgA and IgE antibodies. RESULTS AND CONCLUSIONS: In this study, significant differences were observed in the IgM, IgA and IgE response between the study groups. High IgA and IgE OD ratios in secondary dengue cases were found. The usefulness of serotype specific IgM antibody detection is also analyzed and discussed. A priority for future dengue research in terms of protection, recovery of infection and immunopathogenesis is to elucidate the role of these immunoglobulins. The cross reactivity response to IgM between dengue virus serotypes in primary and secondary cases should also be more studied.
Authors: Sergio I de la Cruz-Hernández; Hilario Flores-Aguilar; Silvia González-Mateos; Irma López-Martinez; Celia Alpuche-Aranda; Juan E Ludert; Rosa M del Angel Journal: Am J Trop Med Hyg Date: 2013-01-21 Impact factor: 2.345
Authors: Amanda E Calvert; Karen L Boroughs; Janeen Laven; Janae L Stovall; Betty E Luy; Olga I Kosoy; Claire Y-H Huang Journal: J Clin Microbiol Date: 2018-05-25 Impact factor: 5.948
Authors: Amanda E Calvert; Kalanthe Horiuchi; Karen L Boroughs; Yee T Ong; Kimberly M Anderson; Brad J Biggerstaff; Mars Stone; Graham Simmons; Michael P Busch; Claire Y-H Huang Journal: J Clin Microbiol Date: 2021-07-19 Impact factor: 5.948
Authors: Maria G Guzman; Thomas Jaenisch; Roger Gaczkowski; Vo Thi Ty Hang; Shamala Devi Sekaran; Axel Kroeger; Susana Vazquez; Didye Ruiz; Eric Martinez; Juan C Mercado; Angel Balmaseda; Eva Harris; Efren Dimano; Prisca Susan A Leano; Sutee Yoksan; Elci Villegas; Herminia Benduzu; Iris Villalobos; Jeremy Farrar; Cameron P Simmons Journal: PLoS Negl Trop Dis Date: 2010-08-31
Authors: Thanh Nga T Tran; Peter J de Vries; Lan Phuong Hoang; Giao T Phan; Hung Q Le; Binh Q Tran; Chi Mai T Vo; Nam V Nguyen; Piet A Kager; Nico Nagelkerke; Jan Groen Journal: BMC Infect Dis Date: 2006-01-25 Impact factor: 3.090