Y H Kim1, Y S Kim, S S Kang, H S Noh, H J Kim, G J Cho, W S Choi. 1. Department of Anatomy and Neurobiology, College of Medicine, Institute of Health Science, Gyeongsang National University, Jinju, South Korea.
Abstract
AIMS/HYPOTHESIS: The present study aimed to investigate the expression levels of and the relationship between 14-3-3 zeta and protein kinase C (PKC) in the retina of early diabetes. METHODS: Changes in the expression levels of, and interaction between, 14-3-3 zeta and PKC were investigated by Northern and Western blot analyses, immunoprecipitation and double immunostaining in the retina of diabetic rats after 6 weeks of diabetes. PKC activity was examined using a PKC assay. RESULTS: In the diabetic retina, the molecular levels of 14-3-3 zeta were reduced, while those of PKC beta and zeta were increased. Direct interaction between 14-3-3 zeta and PKC was markedly decreased in the retina after 6 weeks of diabetes, while PKC activity was increased. CONCLUSIONS/ INTERPRETATION: These findings show that a reduction in 14-3-3 zeta can induce PKC activation, suggesting that this is a main cause of visual dysfunction in the retina during diabetes.
AIMS/HYPOTHESIS: The present study aimed to investigate the expression levels of and the relationship between 14-3-3 zeta and protein kinase C (PKC) in the retina of early diabetes. METHODS: Changes in the expression levels of, and interaction between, 14-3-3 zeta and PKC were investigated by Northern and Western blot analyses, immunoprecipitation and double immunostaining in the retina of diabeticrats after 6 weeks of diabetes. PKC activity was examined using a PKC assay. RESULTS: In the diabetic retina, the molecular levels of 14-3-3 zeta were reduced, while those of PKC beta and zeta were increased. Direct interaction between 14-3-3 zeta and PKC was markedly decreased in the retina after 6 weeks of diabetes, while PKC activity was increased. CONCLUSIONS/ INTERPRETATION: These findings show that a reduction in 14-3-3 zeta can induce PKC activation, suggesting that this is a main cause of visual dysfunction in the retina during diabetes.
Authors: K A Birch; W F Heath; R N Hermeling; C M Johnston; L Stramm; C Dell; C Smith; J R Williamson; A Reifel-Miller Journal: Diabetes Date: 1996-05 Impact factor: 9.461
Authors: Mercedes Pozuelo Rubio; Kathryn M Geraghty; Barry H C Wong; Nicola T Wood; David G Campbell; Nick Morrice; Carol Mackintosh Journal: Biochem J Date: 2004-04-15 Impact factor: 3.857
Authors: Todd E Fox; Megan M Young; Michelle M Pedersen; Sarah Giambuzzi-Tussey; Mark Kester; Thomas W Gardner Journal: Am J Physiol Endocrinol Metab Date: 2011-01-04 Impact factor: 4.310
Authors: Shivangi M Inamdar; Colten K Lankford; Joseph G Laird; Gulnara Novbatova; Nicole Tatro; S Scott Whitmore; Todd E Scheetz; Sheila A Baker Journal: Exp Eye Res Date: 2018-02-24 Impact factor: 3.467
Authors: Na Eun Lee; Yeon Jeong Park; In Young Chung; Seong Wook Seo; Jong Moon Park; Ji Myung Yoo; Jun Kyoung Song Journal: Korean J Ophthalmol Date: 2011-01-17