Literature DB >> 15907694

Isolation and characterization of an Arabidopsis thaliana knockout line for phospholipid: diacylglycerol transacylase gene (At5g13640).

Vandana Mhaske1, Khedidja Beldjilali, John Ohlrogge, Mike Pollard.   

Abstract

To more fully understand the function of phospholipid: diacylglycerol acyltransferase (PDAT, EC 2.3.1.158) in plants we have isolated and characterized a knockout mutant of Arabidopsis thaliana L. which has a T-DNA insertion in PDAT locus At5g13640. Lipid analysis was conducted on these plants to assess the contribution of the PDAT gene to lipid composition. The fatty acid content and composition in seeds do not show significant changes in the mutant. This is a contrary situation to yeast where PDAT is a major contributor to triacylglycerol (TAG) accumulation in exponential growth phase. The results indicate that PDAT activity encoded by At5g13640 is not a major determinant of TAG synthesis in Arabidopsis seeds.

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Year:  2005        PMID: 15907694     DOI: 10.1016/j.plaphy.2005.01.013

Source DB:  PubMed          Journal:  Plant Physiol Biochem        ISSN: 0981-9428            Impact factor:   4.270


  44 in total

1.  ABI4 activates DGAT1 expression in Arabidopsis seedlings during nitrogen deficiency.

Authors:  Yang Yang; Xiangchun Yu; Lianfen Song; Chengcai An
Journal:  Plant Physiol       Date:  2011-04-22       Impact factor: 8.340

2.  Acyl-lipid metabolism.

Authors:  Yonghua Li-Beisson; Basil Shorrosh; Fred Beisson; Mats X Andersson; Vincent Arondel; Philip D Bates; Sébastien Baud; David Bird; Allan Debono; Timothy P Durrett; Rochus B Franke; Ian A Graham; Kenta Katayama; Amélie A Kelly; Tony Larson; Jonathan E Markham; Martine Miquel; Isabel Molina; Ikuo Nishida; Owen Rowland; Lacey Samuels; Katherine M Schmid; Hajime Wada; Ruth Welti; Changcheng Xu; Rémi Zallot; John Ohlrogge
Journal:  Arabidopsis Book       Date:  2010-06-11

3.  Development and analysis of a highly flexible multi-gene expression system for metabolic engineering in Arabidopsis seeds and other plant tissues.

Authors:  Jay Shockey; Catherine Mason; Matthew Gilbert; Heping Cao; Xiangjun Li; Edgar Cahoon; John Dyer
Journal:  Plant Mol Biol       Date:  2015-08-09       Impact factor: 4.076

4.  Acyl-Trafficking During Plant Oil Accumulation.

Authors:  Guanqun Chen; Helen K Woodfield; Xue Pan; John L Harwood; Randall J Weselake
Journal:  Lipids       Date:  2015-10-12       Impact factor: 1.880

5.  The Plastid Lipase PLIP1 Is Critical for Seed Viability in diacylglycerol acyltransferase1 Mutant Seed.

Authors:  Karanbir Aulakh; Timothy P Durrett
Journal:  Plant Physiol       Date:  2019-06-20       Impact factor: 8.340

6.  Genome-wide analysis of PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE (PDAT) genes in plants reveals the eudicot-wide PDAT gene expansion and altered selective pressures acting on the core eudicot PDAT paralogs.

Authors:  Xue Pan; Fred Y Peng; Randall J Weselake
Journal:  Plant Physiol       Date:  2015-01-13       Impact factor: 8.340

7.  Oil-Producing Metabolons Containing DGAT1 Use Separate Substrate Pools from those Containing DGAT2 or PDAT.

Authors:  Anushobha Regmi; Jay Shockey; Hari Kiran Kotapati; Philip D Bates
Journal:  Plant Physiol       Date:  2020-07-30       Impact factor: 8.340

8.  Metabolic network fluxes in heterotrophic Arabidopsis cells: stability of the flux distribution under different oxygenation conditions.

Authors:  Thomas C R Williams; Laurent Miguet; Shyam K Masakapalli; Nicholas J Kruger; Lee J Sweetlove; R George Ratcliffe
Journal:  Plant Physiol       Date:  2008-07-30       Impact factor: 8.340

9.  System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolism.

Authors:  Mingjie Chen; Brian P Mooney; Martin Hajduch; Trupti Joshi; Mingyi Zhou; Dong Xu; Jay J Thelen
Journal:  Plant Physiol       Date:  2009-03-11       Impact factor: 8.340

10.  Simple methods to detect triacylglycerol biosynthesis in a yeast-based recombinant system.

Authors:  Rodrigo M P Siloto; Martin Truksa; Xiaohua He; Thomas McKeon; Randall J Weselake
Journal:  Lipids       Date:  2009-09-10       Impact factor: 1.880

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