Literature DB >> 1590751

Characterization of functional human erythrocyte-type glucose transporter (GLUT1) expressed in insect cells using a recombinant baculovirus.

C K Yi1, B M Charalambous, V C Emery, S A Baldwin.   

Abstract

The human erythrocyte-type glucose transporter (GLUT1) has been abundantly expressed in insect cells by using a recombinant baculovirus. At 4 days after infection with the virus, the insect cell-surface and intracellular membranes were found to contain greater than 200 pmol of D-glucose-sensitive binding sites for the transport inhibitor cytochalasin B per mg of protein. The characteristics of binding were identical with those of the erythrocyte transporter, although the two proteins differed substantially in apparent Mr, probably as a result of glycosylation differences.

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Year:  1992        PMID： 1590751      PMCID： PMC1130932          DOI： 10.1042/bj2830643

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  Reconstitution and purification of the D-glucose transporter from human erythrocytes.

Authors:  M Kasahara; P C Hinkle
Journal:  J Biol Chem       Date:  1977-10-25       Impact factor: 5.157

Review 2.  Homologies between sugar transporters from eukaryotes and prokaryotes.

Authors:  S A Baldwin; P J Henderson
Journal:  Annu Rev Physiol       Date:  1989       Impact factor: 19.318

3.  The human glucose transporter can insert posttranslationally into microsomes.

Authors:  M Mueckler; H F Lodish
Journal:  Cell       Date:  1986-02-28       Impact factor: 41.582

4.  Peptide-specific antibodies as probes of the orientation of the glucose transporter in the human erythrocyte membrane.

Authors:  A Davies; K Meeran; M T Cairns; S A Baldwin
Journal:  J Biol Chem       Date:  1987-07-05       Impact factor: 5.157

5.  Detection and purification of a recombinant human B lymphotropic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization.

Authors:  M C Fung; K Y Chiu; T Weber; T W Chang; N T Chang
Journal:  J Virol Methods       Date:  1988-01       Impact factor: 2.014

6.  Sequence and structure of a human glucose transporter.

Authors:  M Mueckler; C Caruso; S A Baldwin; M Panico; I Blench; H R Morris; W J Allard; G E Lienhard; H F Lodish
Journal:  Science       Date:  1985-09-06       Impact factor: 47.728

7.  Structures of the asparagine-linked sugar chain of glucose transporter from human erythrocytes.

Authors:  T Endo; M Kasahara; A Kobata
Journal:  Biochemistry       Date:  1990-10-02       Impact factor: 3.162

8.  Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins.

Authors:  Y Matsuura; R D Possee; H A Overton; D H Bishop
Journal:  J Gen Virol       Date:  1987-05       Impact factor: 3.891

9.  Expression of a functional glucose transporter in Xenopus oocytes.

Authors:  G W Gould; G E Lienhard
Journal:  Biochemistry       Date:  1989-11-28       Impact factor: 3.162

10.  Expression of the human multidrug transporter in insect cells by a recombinant baculovirus.

Authors:  U A Germann; M C Willingham; I Pastan; M M Gottesman
Journal:  Biochemistry       Date:  1990-03-06       Impact factor: 3.162
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  5 in total

1.  Functional expression of rat renal Na/Pi-cotransport (NaPi-2) in Sf9 cells by the baculovirus system.

Authors:  M Fucentese; K Winterhalter; H Murer; J Biber
Journal:  J Membr Biol       Date:  1995-03       Impact factor: 1.843

2.  Domain assembly of the GLUT1 glucose transporter.

Authors:  D L Cope; G D Holman; S A Baldwin; A J Wolstenholme
Journal:  Biochem J       Date:  1994-06-01       Impact factor: 3.857

3.  Expression of the rat GLUT1 glucose transporter in the yeast Saccharomyces cerevisiae.

Authors:  T Kasahara; M Kasahara
Journal:  Biochem J       Date:  1996-04-01       Impact factor: 3.857

Review 4.  The use of baculoviruses as expression vectors.

Authors:  I M Kidd; V C Emery
Journal:  Appl Biochem Biotechnol       Date:  1993 Aug-Sep       Impact factor: 2.926

5.  Physiological characterization of putative high-affinity glucose transport protein Hxt2 of Saccharomyces cerevisiae by use of anti-synthetic peptide antibodies.

Authors:  D L Wendell; L F Bisson
Journal:  J Bacteriol       Date:  1993-12       Impact factor: 3.490
  5 in total

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