OBJECTIVES: To evaluate the antibacterial activity of four endodontic sealers: one epoxy resin sealer (AH Plus), two zinc oxide eugenol (ZOE)-based sealers (Endomèthasone, Pulp Canal Sealer), and one sealer containing both ZOE and orthophenilphenol (Vcanalare). METHODS: A direct contact test (DCT) was performed. A 10 microl suspension of Enterococcus faecalis was placed on the test material 20 min, 24 h and 7 days after mixing. Bacteria were allowed to directly contact the sealers for 1h at 37 degrees C. Bacterial growth was then spectrophotometrically measured every 30 min for 7 h, and again after 24 h as well. RESULTS: All freshly mixed sealers showed complete inhibition of bacterial growth. Similar results were obtained with the 24-h-old samples, with the exception of AH Plus. Vcanalare was the only sealer still inhibiting bacterial growth 7 days after mixing. CONCLUSIONS: The antimicrobial activity of the tested sealers depends on the time interval between mixing and testing. All sealers exhibit bactericidal effect when freshly mixed, but only Vcanalare extended this effect until 7 days after setting.
OBJECTIVES: To evaluate the antibacterial activity of four endodontic sealers: one epoxy resin sealer (AH Plus), two zinc oxide eugenol (ZOE)-based sealers (Endomèthasone, Pulp Canal Sealer), and one sealer containing both ZOE and orthophenilphenol (Vcanalare). METHODS: A direct contact test (DCT) was performed. A 10 microl suspension of Enterococcus faecalis was placed on the test material 20 min, 24 h and 7 days after mixing. Bacteria were allowed to directly contact the sealers for 1h at 37 degrees C. Bacterial growth was then spectrophotometrically measured every 30 min for 7 h, and again after 24 h as well. RESULTS: All freshly mixed sealers showed complete inhibition of bacterial growth. Similar results were obtained with the 24-h-old samples, with the exception of AH Plus. Vcanalare was the only sealer still inhibiting bacterial growth 7 days after mixing. CONCLUSIONS: The antimicrobial activity of the tested sealers depends on the time interval between mixing and testing. All sealers exhibit bactericidal effect when freshly mixed, but only Vcanalare extended this effect until 7 days after setting.
Authors: Carlos Henrique Gomes Martins; Tatiane Cruz Carvalho; Maria Gorete Mendes Souza; Christian Ravagnani; Oscar Peitl; Edgar Dutra Zanotto; Heitor Panzeri; Luciana Assirati Casemiro Journal: J Mater Sci Mater Med Date: 2011-05-10 Impact factor: 3.896
Authors: Milton Carlos Kuga; Gisele Faria; Marcus Vinicius Só; Katia Cristina Keine; Alailson Domingos dos Santos; Marco Antonio Hungaro Duarte; Patricia Maria Poli Kopper Journal: J Appl Oral Sci Date: 2014-04 Impact factor: 2.698
Authors: Alexandra Conca Alves Mozini; Luis P Vansan; Manoel D Sousa Neto; Rosimeire Pietro Journal: Braz J Microbiol Date: 2009-03-01 Impact factor: 2.476