| Literature DB >> 15906007 |
M Amini1, M Pirali Hamedani, M Vosooghi, M Nabavi, A Shafiee.
Abstract
An HPLC method for determination of rofecoxib in human serum is presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of the drug. Rofecoxib and the internal standard were extracted from serum using liquid-liquid extraction. Upon exposure to UV light, the drug was found to undergo a photocyclization reaction, giving a species with high absorbance. Validation of the method has been studied in the concentration range 10-500 ng ml(-1).Entities:
Mesh：
Substances：
Year: 2005 PMID： 15906007 DOI： 10.1007/s00216-005-3249-6
Source DB: PubMed Journal: Anal Bioanal Chem ISSN： 1618-2642 Impact factor: 4.142