| Literature DB >> 15893391 |
Anandi Martin1, Ernesto Montoro, Dihadenys Lemus, Norberto Simboli, Nora Morcillo, Maritza Velasco, José Chauca, Lucía Barrera, Viviana Ritacco, Françoise Portaels, Juan Carlos Palomino.
Abstract
The performance of the nitrate reductase assay was evaluated in a multicenter laboratory study to detect resistance of Mycobacterium tuberculosis to the first-line anti-tuberculosis drugs rifampicin, isoniazid, ethambutol and streptomycin using a set of coded isolates. Compared with the gold standard proportion method on Löwenstein-Jensen medium, the assay was highly accurate in detecting resistance to rifampicin, isoniazid and ethambutol with an accuracy of 98%, 96.6% and 97.9%, respectively. For streptomycin, discrepant results were obtained with an overall accuracy of 85.3%. The assay proved easy to be implemented in countries with limited laboratory facilities.Entities:
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Year: 2005 PMID： 15893391 DOI： 10.1016/j.mimet.2005.03.004
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363