AIMS: To identify a new fungal strain, Hypocrea sp. F000527 producing a trichothecene metabolite, harzianum A, and to evaluate its cytotoxicity to tumour cell lines. METHODS AND RESULTS: A fungal strain, F000527, with cytotoxic activity was identified as a new Hypocrea strain based on morphological characteristics and internal transcribed spacers rDNA sequence data. Harzianum A was isolated from wheat bran culture by 50% acetone extraction, silica gel chromatography, Sephadex LH-20 chromatography and HPLC. The chemical structures were determined by ESI- or HRFAB-MS and (1)H and (13)C-NMR analyses. Harzianum A showed cytotoxicity to HT1080 and HeLa cell lines with IC(50) value of 0.65 and 5.07 Łg ml(-1) respectively. CONCLUSIONS: Harzianum A with a chemical formula of C(23)H(28)O(6) was isolated from a new Hypocrea strain and showed moderate to strong cytotoxicity to human cancer cell lines. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report of the production of cytotoxic harzianum A by a new Hypocrea strain.
AIMS: To identify a new fungal strain, Hypocrea sp. F000527 producing a trichothecene metabolite, harzianum A, and to evaluate its cytotoxicity to tumour cell lines. METHODS AND RESULTS: A fungal strain, F000527, with cytotoxic activity was identified as a new Hypocrea strain based on morphological characteristics and internal transcribed spacers rDNA sequence data. Harzianum A was isolated from wheat bran culture by 50% acetone extraction, silica gel chromatography, Sephadex LH-20 chromatography and HPLC. The chemical structures were determined by ESI- or HRFAB-MS and (1)H and (13)C-NMR analyses. Harzianum A showed cytotoxicity to HT1080 and HeLa cell lines with IC(50) value of 0.65 and 5.07 Łg ml(-1) respectively. CONCLUSIONS:Harzianum A with a chemical formula of C(23)H(28)O(6) was isolated from a new Hypocrea strain and showed moderate to strong cytotoxicity to humancancer cell lines. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report of the production of cytotoxic harzianum A by a new Hypocrea strain.
Authors: R E Cardoza; M G Malmierca; M R Hermosa; N J Alexander; S P McCormick; R H Proctor; A M Tijerino; A Rumbero; E Monte; S Gutiérrez Journal: Appl Environ Microbiol Date: 2011-06-03 Impact factor: 4.792
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Authors: M G Malmierca; R E Cardoza; N J Alexander; S P McCormick; R Hermosa; E Monte; S Gutiérrez Journal: Appl Environ Microbiol Date: 2012-05-04 Impact factor: 4.792
Authors: Sarah Higginbotham; Weng Ruh Wong; Roger G Linington; Carmenza Spadafora; Liliana Iturrado; A Elizabeth Arnold Journal: PLoS One Date: 2014-01-15 Impact factor: 3.240
Authors: Santiago Gutiérrez; Susan P McCormick; Rosa E Cardoza; Hye-Seon Kim; Laura Lindo Yugueros; Martha Marie Vaughan; Guzmán Carro-Huerga; Mark Busman; Luis E Sáenz de Miera; Walter M Jaklitsch; Wen-Ying Zhuang; Chao Wang; Pedro A Casquero; Robert Henry Proctor Journal: Front Microbiol Date: 2021-12-02 Impact factor: 5.640