| Literature DB >> 15889793 |
P A Zhulidov, E A Bogdanova, A S Shcheglov, I A Shagina, L L Vagner, G L Khazpekov, V V Kozhemiako, S A Luk'ianov, D A Shagin.
Abstract
We developed a new method for the preparation of normalized cDNA libraries enriched with full-length sequences. It is based on the properties of the recently characterized duplex-specific nuclease from the hepatopancreas of the Kamchatka crab. The duplex-specific nuclease is thermostable, it effectively cleaves double-stranded DNA and is inactive toward single-stranded DNA (Shagin et al., Genome Res., 2002, vol. 12, pp. 1935-1942). Our method enables the normalization of cDNA samples enriched with full-length sequences without use of laborious and ineffective stages of physical separation. The efficiency of the method was demonstrated in model experiments using cDNA samples from several human tissues.Entities:
Mesh：
Substances：
Year: 2005 PMID： 15889793 DOI： 10.1007/s11171-005-0023-7
Source DB: PubMed Journal: Bioorg Khim ISSN： 0132-3423