| Literature DB >> 15886876 |
Elena Alhaja1, Jaume Adan, Roser Pagan, Francesc Mitjans, Manel Cascalló, Mercè Rodríguez, Veronique Noé, Carlos J Ciudad, Adela Mazo, Senén Vilaró, Jaume Piulats.
Abstract
Recent evidence has established different functions for the tumor suppressor protein, p16(INK4A) aside from controlling the cell cycle. Here we report that cdk4/6 inhibition blocked both human umbilical vein endothelial cells (HUVEC) spreading on a vitronectin matrix and HUVEC migration on vitronectin. p16 can also act as an anti-angiogenic molecule in vitro since HUVEC and HMEC cells transfected with Ad-p16 or treated with Antennapedia p16 peptides are unable to differentiate on a Matrigel matrix. Both, p16, cyclin D1, cdk4 and cdk6 were immuno-colocalized with Ezrin, Rac, Vinculin, alphav-integrin, and FAK proteins in the ruffles and lamellipodia of migratory cells. Our results indicate that p16 is a key component of a new cytoplasmic pathway controlling angiogenesis of endothelial cells via the alphavbeta3-integrin-mediated migration.Entities:
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Year: 2005 PMID： 15886876 DOI： 10.1007/s10456-005-0368-9
Source DB: PubMed Journal: Angiogenesis ISSN： 0969-6970 Impact factor: 9.596