Literature DB >> 15886694

Stable isotope probing - linking microbial identity to function.

Marc G Dumont1, J Colin Murrell.   

Abstract

Stable isotope probing (SIP) is a technique that is used to identify the microorganisms in environmental samples that use a particular growth substrate. The method relies on the incorporation of a substrate that is highly enriched in a stable isotope, such as (13)C, and the identification of active microorganisms by the selective recovery and analysis of isotope-enriched cellular components. DNA and rRNA are the most informative taxonomic biomarkers and (13)C-labelled molecules can be purified from unlabelled nucleic acid by density-gradient centrifugation. The future holds great promise for SIP, particularly when combined with other emerging technologies such as microarrays and metagenomics.

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Year:  2005        PMID： 15886694     DOI： 10.1038/nrmicro1162

Source DB:  PubMed          Journal:  Nat Rev Microbiol        ISSN： 1740-1526            Impact factor:   60.633


  110 in total

1.  High-throughput isotopic analysis of RNA microarrays to quantify microbial resource use.

Authors:  Xavier Mayali; Peter K Weber; Eoin L Brodie; Shalini Mabery; Paul D Hoeprich; Jennifer Pett-Ridge
Journal:  ISME J       Date:  2011-12-08       Impact factor: 10.302

Review 2.  From structure to function: the ecology of host-associated microbial communities.

Authors:  Courtney J Robinson; Brendan J M Bohannan; Vincent B Young
Journal:  Microbiol Mol Biol Rev       Date:  2010-09       Impact factor: 11.056

3.  Tools for the tract: understanding the functionality of the gastrointestinal tract.

Authors:  Petia Kovatcheva-Datchary; Erwin G Zoetendal; Koen Venema; Willem M de Vos; Hauke Smidt
Journal:  Therap Adv Gastroenterol       Date:  2009-07       Impact factor: 4.409

4.  Subpopulation-specific metabolic pathway usage in mixed cultures as revealed by reporter protein-based 13C analysis.

Authors:  Martin Rühl; Wolf-Dietrich Hardt; Uwe Sauer
Journal:  Appl Environ Microbiol       Date:  2011-01-07       Impact factor: 4.792

5.  Design a cadA-targeted DNA probe for screening of potential bacterial cadmium biosorbents.

Authors:  Bulent Icgen; Fadime Yilmaz
Journal:  Environ Sci Pollut Res Int       Date:  2015-11-20       Impact factor: 4.223

6.  Diversity of active aerobic methanotrophs along depth profiles of arctic and subarctic lake water column and sediments.

Authors:  Ruo He; Matthew J Wooller; John W Pohlman; John Quensen; James M Tiedje; Mary Beth Leigh
Journal:  ISME J       Date:  2012-05-17       Impact factor: 10.302

7.  Identification of bacterial micropredators distinctively active in a soil microbial food web.

Authors:  Tillmann Lueders; Reimo Kindler; Anja Miltner; Michael W Friedrich; Matthias Kaestner
Journal:  Appl Environ Microbiol       Date:  2006-08       Impact factor: 4.792

8.  Stable-Isotope Probing-Enabled Cultivation of the Indigenous Bacterium Ralstonia sp. Strain M1, Capable of Degrading Phenanthrene and Biphenyl in Industrial Wastewater.

Authors:  Jibing Li; Chunling Luo; Dayi Zhang; Xixi Cai; Longfei Jiang; Gan Zhang
Journal:  Appl Environ Microbiol       Date:  2019-07-01       Impact factor: 4.792

9.  Identification of acetate-assimilating microorganisms under methanogenic conditions in anoxic rice field soil by comparative stable isotope probing of RNA.

Authors:  Tomoyuki Hori; Matthias Noll; Yasuo Igarashi; Michael W Friedrich; Ralf Conrad
Journal:  Appl Environ Microbiol       Date:  2006-10-27       Impact factor: 4.792

10.  Mutational activation of niche-specific genes provides insight into regulatory networks and bacterial function in a complex environment.

Authors:  Stephen R Giddens; Robert W Jackson; Christina D Moon; Michael A Jacobs; Xue-Xian Zhang; Stefanie M Gehrig; Paul B Rainey
Journal:  Proc Natl Acad Sci U S A       Date:  2007-11-07       Impact factor: 11.205
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