AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC). METHODS: Enzymatic digestion and Ficoll density centrifugation were used to dissociate ICC from the ileal segment of mice. Factors including contamination, Ca(2+), Mg(2+) and collagenase, and stem cell factor, etc., were investigated. ACK2, the antibody of c-kit, was used to identify the cultured ICC. Both light microscope and fluorescence microscope were used to observe the changes of ICC in vitro. RESULTS: The method for dissociation and culture of ICC in vitro was successfully established. After 24 h, cultured ICC exhibited a few axis-cylinders, and longer axis-cylinders were observed to form synapse of each other after 3 d. More widespread connections formed within 7 d in vitro. The changes of its morphologic character were obvious within 7 d; however, there were no obvious morphologic changes after 30 d. CONCLUSION: Many factors can influence the dissociation and culture of ICC.
AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC). METHODS: Enzymatic digestion and Ficoll density centrifugation were used to dissociate ICC from the ileal segment of mice. Factors including contamination, Ca(2+), Mg(2+) and collagenase, and stem cell factor, etc., were investigated. ACK2, the antibody of c-kit, was used to identify the cultured ICC. Both light microscope and fluorescence microscope were used to observe the changes of ICC in vitro. RESULTS: The method for dissociation and culture of ICC in vitro was successfully established. After 24 h, cultured ICC exhibited a few axis-cylinders, and longer axis-cylinders were observed to form synapse of each other after 3 d. More widespread connections formed within 7 d in vitro. The changes of its morphologic character were obvious within 7 d; however, there were no obvious morphologic changes after 30 d. CONCLUSION: Many factors can influence the dissociation and culture of ICC.
Authors: A Epperson; W J Hatton; B Callaghan; P Doherty; R L Walker; K M Sanders; S M Ward; B Horowitz Journal: Am J Physiol Cell Physiol Date: 2000-08 Impact factor: 4.249
Authors: Adam Rich; Steven M Miller; Simon J Gibbons; John Malysz; Joseph H Szurszewski; G Farrugia Journal: Am J Physiol Gastrointest Liver Physiol Date: 2002-10-09 Impact factor: 4.052
Authors: S J Gibbons; A Rich; M A Distad; S M Miller; P F Schmalz; J H Szurszewski; L Sha; P Blume-Jensen; G Farrugia Journal: Neurogastroenterol Motil Date: 2003-12 Impact factor: 3.598