OBJECTIVE: To investigate the protective effect of ischemic preconditioning (IP) in the early phase of reperfusion injury. DESIGN AND METHODS: Control rat lungs were subjected to 3 h of perfusion, whereas the lungs of the ischemia-then-reperfusion (I/R) group were subjected to 2 h of cold ischemia following 30 min of perfusion. IP was performed by two cycles of 5-min ischemia followed by 5 min of reperfusion prior to 2-h cold ischemia. Lipid peroxidation and antioxidant status were determined in tissue samples. RESULTS: Lipid peroxidation and reduced/oxidized glutathione (GSH/GSSG) ratio were increased; antioxidant enzyme activities were decreased in the I/R group whereas lipid peroxidation and GSH/GSSG ratio were decreased; antioxidant enzyme activities were increased in the IP group. CONCLUSION: IP appeared to have a protective effect against reactive oxygen species-mediated I/R injury in isolated rat lung.
OBJECTIVE: To investigate the protective effect of ischemic preconditioning (IP) in the early phase of reperfusion injury. DESIGN AND METHODS: Control rat lungs were subjected to 3 h of perfusion, whereas the lungs of the ischemia-then-reperfusion (I/R) group were subjected to 2 h of cold ischemia following 30 min of perfusion. IP was performed by two cycles of 5-min ischemia followed by 5 min of reperfusion prior to 2-h cold ischemia. Lipid peroxidation and antioxidant status were determined in tissue samples. RESULTS:Lipid peroxidation and reduced/oxidized glutathione (GSH/GSSG) ratio were increased; antioxidant enzyme activities were decreased in the I/R group whereas lipid peroxidation and GSH/GSSG ratio were decreased; antioxidant enzyme activities were increased in the IP group. CONCLUSION: IP appeared to have a protective effect against reactive oxygen species-mediated I/R injury in isolated rat lung.
Authors: Anthony W Castleberry; Mathias Worni; Asishana A Osho; Laurie D Snyder; Scott M Palmer; Ricardo Pietrobon; R Duane Davis; Matthew G Hartwig Journal: Am J Respir Crit Care Med Date: 2013-08-15 Impact factor: 21.405
Authors: Chwen-Lih Chen; Liwen Zhang; Alexander Yeh; Chun-An Chen; Kari B Green-Church; Jay L Zweier; Yeong-Renn Chen Journal: Biochemistry Date: 2007-04-20 Impact factor: 3.162