OBJECTIVE: To determine the roles of insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) in the pathogenesis of osteoarthritis (OA). DESIGN: Cartilage tissues were obtained from the femoral heads of patients with OA, and those from patients with femoral neck fractures were used as a control. The expression of IGFBP-3, -4, and -5 was examined using immunohistochemistry and in situ hybridization, and IGF-I and IGF-I receptors were also immunohistochemically detected. The percentages of positive chondrocytes were determined by counting the total number of chondrocytes over the area of the surface, middle, and deep zones of the cartilage. RESULTS: There was a marked increase in the percentage of positive chondrocytes in all IGFBPs on protein and messenger RNA levels for OA compared to that of the control cartilage. Furthermore, enhanced expression of IGFBPs and the IGF-I/IGF-I receptor was positively correlated with the histologic score for cartilage lesions. CONCLUSION: Up-regulation of IGFBPs as well as IGF-I and its receptor was observed for OA cartilage tissue, suggesting the involvement of IGFBPs in the pathogenesis of OA.
OBJECTIVE: To determine the roles of insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) in the pathogenesis of osteoarthritis (OA). DESIGN:Cartilage tissues were obtained from the femoral heads of patients with OA, and those from patients with femoral neck fractures were used as a control. The expression of IGFBP-3, -4, and -5 was examined using immunohistochemistry and in situ hybridization, and IGF-I and IGF-I receptors were also immunohistochemically detected. The percentages of positive chondrocytes were determined by counting the total number of chondrocytes over the area of the surface, middle, and deep zones of the cartilage. RESULTS: There was a marked increase in the percentage of positive chondrocytes in all IGFBPs on protein and messenger RNA levels for OA compared to that of the control cartilage. Furthermore, enhanced expression of IGFBPs and the IGF-I/IGF-I receptor was positively correlated with the histologic score for cartilage lesions. CONCLUSION: Up-regulation of IGFBPs as well as IGF-I and its receptor was observed for OA cartilage tissue, suggesting the involvement of IGFBPs in the pathogenesis of OA.
Authors: Anja Weimer; Henning Madry; Jagadeesh K Venkatesan; Gertrud Schmitt; Janina Frisch; Anna Wezel; Jochen Jung; Dieter Kohn; Ernest F Terwilliger; Stephen B Trippel; Magali Cucchiarini Journal: Mol Med Date: 2012-05-09 Impact factor: 6.354
Authors: Daniel S Evans; Frederic Cailotto; Neeta Parimi; Ana M Valdes; Martha C Castaño-Betancourt; Youfang Liu; Robert C Kaplan; Martin Bidlingmaier; Ramachandran S Vasan; Alexander Teumer; Gregory J Tranah; Michael C Nevitt; Steven R Cummings; Eric S Orwoll; Elizabeth Barrett-Connor; Jordan B Renner; Joanne M Jordan; Michael Doherty; Sally A Doherty; Andre G Uitterlinden; Joyce B J van Meurs; Tim D Spector; Rik J Lories; Nancy E Lane Journal: Ann Rheum Dis Date: 2014-06-13 Impact factor: 19.103
Authors: Carlos Gonzalez; Kiem G Auw Yang; Joseph H Schwab; James S Fitzsimmons; Monica M Reinholz; Zachary T Resch; Laurie K Bale; Victoria R Clemens; Cheryl A Conover; Shawn W O'Driscoll; Gregory G Reinholz Journal: Growth Horm IGF Res Date: 2009-08-04 Impact factor: 2.372
Authors: W H Busby; S A Yocum; M Rowland; D Kellner; S Lazerwith; F Sverdrup; M Yates; M Radabaugh; D R Clemmons Journal: Osteoarthritis Cartilage Date: 2008-10-18 Impact factor: 6.576
Authors: Anna L Stevens; John S Wishnok; Forest M White; Alan J Grodzinsky; Steven R Tannenbaum Journal: Mol Cell Proteomics Date: 2009-02-04 Impact factor: 5.911
Authors: Manoj Ramachandran; Prim Achan; Donald M Salter; Dan L Bader; Tina T Chowdhury Journal: Arthritis Res Ther Date: 2011-09-13 Impact factor: 5.156
Authors: Giorgio Gasparini; Marco De Gori; Francesco Paonessa; Eusebio Chiefari; Antonio Brunetti; Olimpio Galasso Journal: Arthritis Res Ther Date: 2012-10-04 Impact factor: 5.156